PXD071369

PXD071369 is an original dataset announced via ProteomeXchange.

Title	Identification proteins associated with VgrG3 of Chromobacterium violaceum
Description	Bacteria in polymicrobial communities engage in complex interactions, including interbacterial antagonism. In Chromobacterium violaceum, the Type VI Secretion System (T6SS) serves as a critical antibacterial weapon, but the full set of effectors delivered by this system remains not yet completely defined. To comprehensively identify T6SS effectors, we performed co-immunoprecipitation of VgrG3, one of the six VgrGs encoded by C. violaceum, followed by LC–MS/MS analysis. Comparative proteomics revealed a diverse array of VgrG3-associated proteins, including all six VgrG homologs, phospholipase effectors from the Tle1 and Tle5 families, PAAR-domain proteins, Tap-1–like adaptors, and the Rhs-family protein RhsF. Functional analyses confirmed that RhsF acts as a T6SS-dependent antibacterial toxin neutralized by its cognate immunity protein, RhsFi (CV_1430), forming a toxin-immunity pair. X-ray crystallography of the RhsF-CT/RhsFi complex at 1.85 Å resolution revealed structural features of ADP-ribosyltransferases in RhsF and demonstrated that RhsFi inhibits toxicity by directly occluding the catalytic site. Mutagenesis assays further confirmed that RhsF toxicity depends on a catalytic triad composed of residues R1403, Y1456, and E1497. These findings expand the known repertoire of T6SS toxins in C. violaceum and place RhsF as a newly identified antibacterial effector in this bacterium.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-23_16:23:05.573.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Alves
SpeciesList	scientific name: Chromobacterium violaceum ATCC 12472; NCBI TaxID: NEWT:243365;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-28 07:48:25	ID requested
⏵ 1	2026-08-23 16:23:06	announced

10.1016/j.jbc.2026.113216;

submitter keyword: Chromobacterium violaceum, Type VI Secretion System, Interbacterial competition, Antibacterial effectors

Jose Freire da Silva
contact affiliation	Departamento de Biologia Celular e Molecular e Bioagentes Patogenicos
contact email	jfsneto@usp.br
lab head
Julia Alves
contact affiliation	University of Dundee
contact email	jalves001@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD071369

PRIDE project URI

• PRIDE
  1. PXD071369
     1. Label: PRIDE project
     2. Name: Identification proteins associated with VgrG3 of Chromobacterium violaceum