PXD071366

PXD071366 is an original dataset announced via ProteomeXchange.

Title	CD47 stabilizes ROBO2 to regulate glioblastoma progression by preventing ITCH mediated ubiquitination
Description	CD47 is a key innate immune checkpoint that inhibits phagocytosis by myeloid cells, contributing to immune evasion by cancer cells. Although CD47-blocking antibodies have shown promise in treating various malignancies, their efficacy in glioblastoma (GBM) remains limited, and the cell-intrinsic role of CD47 is poorly understood. In this study, we show that CD47 is highly expressed at the invasive edge of GBM tumors, and its elevated expression correlates with poor patient survival. We demonstrate that CD47 loss impairs GBM cell proliferation, migration, and invasion, independent of immune activity and leads to reduced tumor burden and prolonged survival in vivo. Our study reveals ROBO2 signaling as the downstream effector of CD47 and loss of ROBO2 also attenuates GBM cell proliferation and migration. Importantly, we have uncovered that CD47 stabilizes ROBO2 by sequestering the E3 ubiquitin ligase ITCH, thereby blocking ubiquitination and proteasomal degradation of ROBO2. These findings establish CD47 as a key regulator of GBM cell plasticity, and highlight the therapeutic potential of targeting CD47-ROBO2 signaling in GBM.
HostingRepository	PRIDE
AnnounceDate	2026-03-06
AnnouncementXML	Submission_2026-03-05_16:37:29.761.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ghizal Siddiqui
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-28 06:43:17	ID requested
⏵ 1	2026-03-05 16:37:30	announced

Dataset with its publication pending

submitter keyword: CD47

Glioblastoma

ROBO2 signaling

Ubiquitination

Q Exactive HF Hybrid Quadrupole-Orbitrap

E3 ubiquitin ligase ITCH

Nirmal Robinson
contact affiliation	Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, SA, Australia Adelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, SA, Australia
contact email	nirmal.robinson@unisa.edu.au
lab head
Ghizal Siddiqui
contact affiliation	Monash University
contact email	ghizal.siddiqui@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD071366

PRIDE project URI

• PRIDE
  1. PXD071366
     1. Label: PRIDE project
     2. Name: CD47 stabilizes ROBO2 to regulate glioblastoma progression by preventing ITCH mediated ubiquitination