PXD071320

PXD071320 is an original dataset announced via ProteomeXchange.

Title	Autonomous biogenesis of all thirty proteins of the E. coli translation machineryk
Description	The cell-free biogenesis of the protein translation machinery is essential for the creation of a self-regenerating synthetic cell. Here, we demonstrate the autonomous and simultaneous biogenesis of all thirty proteins of the translation machinery of E. coli in a reconstituted transcription-translation system. We first established self-regeneration of every translation protein by determining the threshold concentration required for its own synthesis from a synthetic gene coding for the protein, thereby demonstrating the functionality of all nascent proteins, separately. Simultaneous biogenesis of multiple translation proteins at their threshold resulted in delayed synthesis below detection levels. To achieve self-regeneration of multiple translation proteins, we induced boundary-free compartmentalization of the reaction by immobilizing the genes on a surface at high density. The co-localization of genes, molecular machinery, messenger RNA and nascent proteins at the surface created sufficient conditions to catalyze the simultaneous self-regeneration of sub-groups up to all thirty translation proteins, as measured by total internal reflection fluorescence on the surface. Our approach provides mechanistic insight and presents a general methodology for the biogenesis of cellular machines toward autonomous synthetic systems.
HostingRepository	PRIDE
AnnounceDate	2025-12-09
AnnouncementXML	Submission_2025-12-09_01:41:02.653.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amir Prior
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480; timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-27 04:24:02	ID requested
⏵ 1	2025-12-09 01:41:03	announced

Dataset with its publication pending

submitter keyword: cell-free biogenesis,E. coli, LC-MS/MS

Roy H. Bar-Ziv
contact affiliation	Department of Chemical and Biological Physics, The Weizmann Institute of Science, Rehovot, Israel
contact email	roy.bar-ziv@weizmann.ac.il
lab head
Amir Prior
contact affiliation	Weizmann Institute of Science
contact email	amir.prior@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD071320

PRIDE project URI

• PRIDE
  1. PXD071320
     1. Label: PRIDE project
     2. Name: Autonomous biogenesis of all thirty proteins of the E. coli translation machineryk