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PXD071288
PXD071288 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization, Antioxidant Effects of Lycium ruthenicum Murr. Polysaccharides and its Protective Effects Against Oxaliplatin-Induced Peripheral Neurotoxicity by Inhibiting the RAS-RAF-MEK-ERK Signaling Pathway |
| Description | Oxaliplatin is a frequently used chemotherapeutic agent; however, oxaliplatin-induced peripheral neuropathy (OIPN) is a prevalent adverse effect for which no effective treatment currently exists. Lycium ruthenicum Murr. (LR), a traditional Chinese medicinal herb also consumed as food, is rich in polysaccharides. However, the influence of alcohol precipitation concentration on the structure and bioactivities of polysaccharides from Lycium ruthenicum Murr (LRPs) remains inadequately understood, nor is its effect on OIPN. In this study, three novel LRPs (LRP-20, LRP-50, and LRP-80) were isolated using 20%, 50% and 80% ethanol, respectively, from crude LRPs extracted with hot water. LRP-20 (5846 Da), LRP-50 (7327 Da), and LRP-80 (9980 Da) differed in monosaccharide composition and content. FT-IR, SEM, XRD, and thermal stability assessments revealed distinct differences in the structural properties among the LRPs. In vitro tests found LRP-20 exhibited the highest antioxidant activity, relevant to OIPN's main pathogenic mechanisms. In OIPN rat models, LRP-20 potentially alleviated OIPN by augmenting antioxidant capacity and attenuating inflammatory responses mediated through the RAS-RAF-MEK-ERK signaling pathway. This research demonstrated that ethanol fractional precipitation can initially separate polysaccharides according to the structure, which exhibits strong correlation with the biological activities, and providing an approach for the further development of LPRs in the field of peripheral nerve protection. |
| HostingRepository | iProX |
| AnnounceDate | 2025-11-26 |
| AnnouncementXML | Submission_2026-02-02_23:19:48.980.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Limei Wen |
| SpeciesList | scientific name: Rattus norvegicus; NCBI TaxID: 10116; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-26 17:34:20 | ID requested | |
| 1 | 2025-11-26 17:34:38 | announced | |
| ⏵ 2 | 2026-02-02 23:19:49 | announced | 2026-02-03: Add info. |
Publication List
| Wen L, Xue T, Guo Y, Wang Y, Hou Q, Geng R, Ma C, Ding N, Chen J, Xue Q, Hu J, Yang J, Characterization, antioxidant effects of Lycium ruthenicum Murr. Polysaccharides and protective effects against Oxaliplatin-induced peripheral neurotoxicity through inhibition of the RAS - RAF - MEK - ERK signaling pathway. Food Res Int, 226():118115(2026) [pubmed] |
Keyword List
| submitter keyword: oxaliplatin-induced peripheral neuropathy, antioxidant activity, Lycium ruthenicum Murr., Polysaccharides, RAS-RAF1-MEK-ERK signaling pathway |
Contact List
| Jianhua Yang | |
|---|---|
| contact affiliation | Department of Pharmacy, The First Affiliated Hospital of Xinjiang Medical University |
| contact email | yjh_yfy@163.com |
| lab head | |
| Limei Wen | |
| contact affiliation | Department of Pharmacy, The First Affiliated Hospital of Xinjiang Medical University |
| contact email | wendywenlimei@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
