PXD071282 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Deep Top-Down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry: Identification of 5700 Proteoforms from the Escherichia coli Proteome |
| Description | We present an orthogonal multidimensional separation platform that couples size exclusion chromatography (SEC) and RPLC based protein prefractionation to CZE-MS/MS for deep top-down proteomics of Escherichia coli. The platform generated high peak capacity (∼4000) for separation of intact proteins, leading to the identification of 5700 proteoforms from the Escherichia coli proteome. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-15 |
| AnnouncementXML | Submission_2025-12-15_08:36:15.334.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kun Li |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-26 15:24:48 | ID requested | |
| ⏵ 1 | 2025-12-15 08:36:16 | announced | |
Publication List
| 10.1021/acs.analchem.8b00693; |
| 10.1021/ACS.ANALCHEM.8B00693; |
| McCool EN, Lubeckyj RA, Shen X, Chen D, Kou Q, Liu X, Sun L, Deep Top-Down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry: Identification of 5700 Proteoforms from the Escherichia coli Proteome. Anal Chem, 90(9):5529-5533(2018) [pubmed] |
Keyword List
| submitter keyword: Top-down proteomics, RPLC, SEC, E.coli |
Contact List
| Liangliang Sun |
|---|
| contact affiliation | Michigan State University |
| contact email | lsun@chemistry.msu.edu |
| lab head | |
| Kun Li |
|---|
| contact affiliation | Tulane University |
| contact email | kli7@tulane.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD071282 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071282
- Label: PRIDE project
- Name: Deep Top-Down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry: Identification of 5700 Proteoforms from the Escherichia coli Proteome
