PXD071267

PXD071267 is an original dataset announced via ProteomeXchange.

Title	Identification of UFM1 candidate targets from mouse brain
Description	Genetic variants that hinder post-translational protein modifications by the ubiquitin-like modifier UFM1 (UFMylation) cause encephalopathies. UFMylation regulates endoplasmic reticulum (ER) homeostasis, but how UFMylation-deficiencies cause selective neurological defects is unknown. In the framework of our studies on two types of UFMylation pathologies, UFM1 loss and expression of a pathogenic UFM1-R81C variant, we conducted a pilot screen for neuronal UFM1 candidate targets in the mouse brain. We used anti-UFM1 antibodies to affinity-purify potentially UFM1-modified proteins from mouse brain and identified them via a gel-based proteomic approach. Apart from free UFM1, UBA5 and UFC1 as major members of the UFMylation machinery were found to be enriched, providing proof-of-concept to our approach. Among the ~300 potentially UFMylated proteins exclusively identified in the anti-UFM1 antibody sample, we found several guanyl-nucleotide exchange factors, including DOCK4 as the most prominent candidate target. While the UFMylation of DOCK4 remains to be established, this candidate is particularly interesting as it is associated with neurodevelopmental delay and microcephaly. Thus, our findings may open a new area of future investigation toward the role UFMylation in neuronal biology and pathology.
HostingRepository	PRIDE
AnnounceDate	2026-01-28
AnnouncementXML	Submission_2026-01-28_09:19:07.334.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-26 08:34:28	ID requested
⏵ 1	2026-01-28 09:19:07	announced

Dataset with its publication pending

submitter keyword: gel electrophoresis, brain, affinity purification, mass spectrometry, encephalopathy,Ubiquitin-like modifier, UFM1

Prof. Dr. Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen Germany
contact email	jahn@mpinat.mpg.de
lab head
Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen, Germany
contact email	jahn@mpinat.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD071267

PRIDE project URI

• PRIDE
  1. PXD071267
     1. Label: PRIDE project
     2. Name: Identification of UFM1 candidate targets from mouse brain