PXD071250 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative proteome of membrane proteome from Lactobacillus fermentum MI37 |
| Description | Recent findings have positioned the gut microbiota as a promising target to enhance cancer immunotherapy. However, the specific microbial molecules that drive anti-tumor immunity remain unclear. Here, we identify a human-derived strain, Lactobacillus fermentum MI37, that potentiates anti-cancer immune responses and synergizes with anti-PD-1 immune checkpoint blockade. MI37 robustly induces IFN-γ production by splenocytes, with minimal IL-10 induction, and enhances cytotoxic T lymphocyte infiltration and inflammatory gene expression within tumors in a syngeneic mouse model. Remarkably, heat-killed MI37 retains its immunostimulatory effects and anti-tumor efficacy. Fractionation experiments implicate lipoteichoic acid (LTA) as the active component, and LTA mimics the effects of MI37 on IFN-γ production and CD8⁺ T cell activation. Genomic and proteomic profiling reveals MI37-specific pathways involved in teichoic acid biosynthesis, including a unique sulfatase N-terminal domain-containing protein with an LTA synthase domain. These findings highlight the potential of LTA-producing probiotics as microbial adjuvants to improve the efficacy of cancer immunotherapy. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-25 |
| AnnouncementXML | Submission_2026-05-24_16:32:30.742.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kyunggon Kim |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: NEWT:562; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-25 23:39:05 | ID requested | |
| ⏵ 1 | 2026-05-24 16:32:31 | announced | |
Publication List
| 10.1016/j.isci.2026.115642; |
| Kim TY, Kim S, Lee S, Lee SH, Yu J, Kim K, Kweon MN, T cells for enhanced anti-cancer immunity. iScience, 29(5):115642(2026) [pubmed] |
Keyword List
| submitter keyword: anti-cancer,Lactobacillus fermentum MI37, LC-MS, IFN-gamma, membrane proteome |
Contact List
| Kyunggon Kim |
|---|
| contact affiliation | Department of Medical Informatics and Statistics, BK21 Project, Asan Medical Center, Asan Institute for Life Sciences, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. |
| contact email | kkkon1@amc.seoul.kr |
| lab head | |
| Kyunggon Kim |
|---|
| contact affiliation | Asan Medical Center |
| contact email | kimkyunggon@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071250
- Label: PRIDE project
- Name: Comparative proteome of membrane proteome from Lactobacillus fermentum MI37
