PXD071225

PXD071225 is an original dataset announced via ProteomeXchange.

Title	Cellular senescence escape and antiviral response discriminate glioblastoma from lower grade gliomas – a bioinformatic meta-analysis
Description	Cellular senescence plays a negative prognostic role in glioblastoma (GB). However, the specific cell types with senescent features and molecular mechanisms by which these senescent cells contribute to the pathogenesis of GB are unknown. To identify specific senescent cell subpopulations in GB and their functional impact on surrounding cells, we performed bioinformatic analyses of publicly available omics datasets, specifically transcriptome, single-cell transcriptome, and proteome data, obtained directly from patients with GB. Markers of cellular senescence were defined based on publicly available transcriptome data comparing senescent and proliferating cells. They were subsequently used to verify the presence of senescent cells in GB datasets. By applying this systematically curated set of common senescence markers together with the expression of senescence-associated secretory phenotype (SASP), extracellular matrix (ECM) components, and cyclin-dependent kinase inhibitors, we identified GB-associated senescent cells in clusters phenotypically corresponding to subpopulations of the developing brain, including radial glia, endothelial cells, and immature astrocytes. We characterized the communication network of senescent cells with other cells in the tumor tissue, showing that senescent cells communicate primarily with tumor-associated macrophages to suppress immune response. Notably, processes that distinguish GB from less malignant types of gliomas include cellular senescence and pathways associated with antiviral response. To investigate the effect of chemotherapy on the development of secondary therapy-induced senescence, we performed a proteomic analysis of an in vitro 3D model of GB cell spheroids induced to senescence by temozolomide. Results indicate that primary and secondary senescence in GB shares similar phenotypic features. Pathways that are associated with unique GB aggressiveness and therapy resistance are upregulated after therapy, which can promote even more aggressive behavior of the recurrent tumor. The data underscores that senescent cells contribute significantly to the malignant manifestations of GB, supporting the emerging idea that senescent cells are a promising target for adjuvant senolytic therapy of GB.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_18:13:26.523.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michaela Sadibolova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-25 15:47:36	ID requested
⏵ 1	2026-06-07 18:13:27	announced

Maurencova D, Sadibolova M, Zarska M, Liblova Z, Novak J, Kroupova J, Vasicova P, Bartek J, Hodny Z, Cellular senescence escape and antiviral response discriminate glioblastoma from lower-grade gliomas. Neurooncol Adv, 8(1):vdag122(2026) [pubmed]

10.1093/noajnl/vdag122;

submitter keyword: None

Zdenek Hodny
contact affiliation	Institute of Molecular Genetics of the Czech Academy of Sciences
contact email	zdenek.hodny@img.cas.cz
lab head
Michaela Sadibolova
contact affiliation	University Hospital Hradec Kralove
contact email	michaela.sadibolova@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD071225

PRIDE project URI

• PRIDE
  1. PXD071225
     1. Label: PRIDE project
     2. Name: Cellular senescence escape and antiviral response discriminate glioblastoma from lower grade gliomas – a bioinformatic meta-analysis