PXD071225

PXD071225 is an original dataset announced via ProteomeXchange.

Title	Cellular senescence escape and antiviral response discriminate glioblastoma from lower grade gliomas
Description	Cellular senescence is a negative prognostic indicator in glioblastoma (GB). However, the specific cell types exhibiting senescence and the molecular mechanisms by which senescent cells (SCs) contribute to GB pathogenesis remain unknown. We performed multi-omics integration of publicly available GB patient-derived datasets to identify SCs and their functional impact on GB pathogenesis. We created a transcriptomic definition of SCs to verify their presence in GB datasets. Next, we analyzed transcriptomic profiles of GB and low-grade gliomas to reveal key features of GB aggressiveness. The GB patients’ phosphoproteome was analyzed with a focus on key regulators of senescence. To assess chemotherapy-evoked secondary senescence, we performed a proteomic analysis of temozolomide-induced senescence in human GB spheroids. We identified GB-associated SCs in clusters radial glia, endothelial cells, and immature astrocytes localized primarily in the hypoxic zones. Notably, we identified senescence-escape features and tumor antiviral responses as processes distinguishing GB from low-grade gliomas. In GB samples, we detected inhibitory phosphorylation of p21 and p27 proteins and active PI3K signaling, which can lead to senescence escape and belong to the typical manipulation arsenal of herpesviruses. Our proteomic analysis of temozolomide-induced senescent GB cells reveals that primary (pre-radiochemotherapy) and secondary senescence in GB share similar phenotypic features. Pathways associated with GB aggressiveness are upregulated after therapy, which can promote more aggressive behavior of recurrent tumors. Our data indicate that senescence and viral reactivation may fuel GB progression, including recurrence, suggesting that senolytics and antiviral drugs are potential therapeutic avenues.
HostingRepository	PRIDE
AnnounceDate	2026-06-12
AnnouncementXML	Submission_2026-06-12_00:19:14.170.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michaela Sadibolova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-25 15:47:36	ID requested
1	2026-06-07 18:13:27	announced
⏵ 2	2026-06-12 00:19:15	announced	2026-06-12: Updated project metadata.

Maurencova D, Sadibolova M, Zarska M, Liblova Z, Novak J, Kroupova J, Vasicova P, Bartek J, Hodny Z, Cellular senescence escape and antiviral response discriminate glioblastoma from lower-grade gliomas. Neurooncol Adv, 8(1):vdag122(2026) [pubmed]

10.1093/noajnl/vdag122;

submitter keyword: None

Zdenek Hodny
contact affiliation	Institute of Molecular Genetics of the Czech Academy of Sciences
contact email	zdenek.hodny@img.cas.cz
lab head
Michaela Sadibolova
contact affiliation	University Hospital Hradec Kralove
contact email	michaela.sadibolova@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD071225

PRIDE project URI

• PRIDE
  1. PXD071225
     1. Label: PRIDE project
     2. Name: Cellular senescence escape and antiviral response discriminate glioblastoma from lower grade gliomas