PXD071213

PXD071213 is an original dataset announced via ProteomeXchange.

Title	Analysis of Zyxin's interactome and phosphosites in early Xenopus laevis development by Data-Independent Acquisition proteomics
Description	Protein complexes are key players in development, functioning as molecular machines whose proper assembly is crucial for numerous cellular processes. Their formation at the correct time and location is facilitated by specialized scaffold proteins. A prominent example is the mechanosensitive cytoskeletal protein zyxin. Zyxin is of particular interest due to its role in assembling actin filaments within focal adhesion complexes and transducing signals from the cytoskeleton to the genetic apparatus. In this study, we employed co-immunoprecipitation coupled with mass spectrometry to analyze changes in zyxin-associated protein complexes from lysates of Xenopus frog embryos across three early developmental stages: early gastrulation, early neurulation, and late neural fold stages. Our analysis revealed stage-specific alterations in the isoform repertoires of several established zyxin partners, including components of the focal adhesion complex, transcriptional regulators, and proteins involved in its phosphorylation. We also identified changes in the phosphorylation patterns of different zyxin isoforms. Collectively, these findings provide a valuable resource for elucidating the function of the scaffold protein zyxin and its role in embryonic tissue development. Furthermore, the identification of kinases and caspases within the zyxin interactome suggests its involvement in key signaling pathways related to cell survival and apoptosis. Finally, confirmed interactions with partners such as 14-3-3 proteins, Yap, and TEF-1 highlight the multifaceted role of zyxin in cellular regulation and its potential as a therapeutic target.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:13:03.653.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rustam Ziganshin
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: NEWT:8355;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-25 13:26:29	ID requested
⏵ 1	2026-02-01 16:13:04	announced

Parshina EA, Ziganshin RH, Zaraisky AG, Martynova NY, Development. Int J Mol Sci, 27(2):(2026) [pubmed]

10.3390/ijms27020738;

submitter keyword: zyxin,Xenopus laevis. LC-MS

Zaraisky A.G.
contact affiliation	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
contact email	azaraisky@yahoo.com
lab head
Rustam Ziganshin
contact affiliation	Shemyakin-Ovchinnikov Institute of bioorganic chemistry Russian Academy of Sciences
contact email	rustam.ziganshin@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071213
     1. Label: PRIDE project
     2. Name: Analysis of Zyxin's interactome and phosphosites in early Xenopus laevis development by Data-Independent Acquisition proteomics