PXD071198

PXD071198 is an original dataset announced via ProteomeXchange.

Title	Identification of salicylic acid (SA)- and pathogen-responsive ubiquitin-binding proteins in Arabidopsis thaliana
Description	The ubiquitin-proteasome system (UPS) is a key regulatory mechanism in plant immunity. Target proteins are labelled with ubiquitin chains that form distinct topological structures to modulate their activity and stability. Here, we identified ubiquitin-binding proteins (UBPs) that specifically interact with M1-, K48-, and K63-linked ubiquitin chains in response to SA or infection with the bacterial pathogen Pseudomonas syringae pv. maculicola ES4326 (Psm). Four-week-old plants were sprayed with 0.5 mM SA or inoculated with Psm ES4326, samples were harvested after 24 hours post-treatment. For the M1- and K48-linked ubiquitin chain pulldown assays, monoubiquitin (Ub) was used as a control. For the K63-linked ubiquitin chain pulldown, empty agarose beads (Beads) served as the control. In total three independent biological repeats were collected.
HostingRepository	PRIDE
AnnounceDate	2026-02-12
AnnouncementXML	Submission_2026-02-12_09:07:24.432.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhishuo Wang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-25 09:03:59	ID requested
⏵ 1	2026-02-12 09:07:24	announced

Dataset with its publication pending

submitter keyword: None

Steven Spoel
contact affiliation	Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh
contact email	Steven.Spoel@ed.ac.uk
lab head
Zhishuo Wang
contact affiliation	University of Edinburgh
contact email	zhishuo.wang@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD071198

PRIDE project URI

• PRIDE
  1. PXD071198
     1. Label: PRIDE project
     2. Name: Identification of salicylic acid (SA)- and pathogen-responsive ubiquitin-binding proteins in Arabidopsis thaliana