PXD071193

PXD071193 is an original dataset announced via ProteomeXchange.

Title	Functional atlas of vertebrate viral RNA elements that stabilize RNA and enhance translation
Description	Viruses encode diverse regulatory elements, but their breadth and mechanisms remain poorly defined. To address this gap, we performed massively parallel reporter assays spanning ~200,000 genomic segments from 297 vertebrate-infecting viral genera. We identified numerous viral elements that enhance RNA stability and translation through TENT4-mediated mixed tailing, distributed across 18 genera and grouped into six distinct subclasses, indicating extensive convergent evolution. We also found diverse TENT4-independent elements acting through alternative pathways. One of such elements, Pt1 from Potamipivirus, stabilizes linear mRNA to levels comparable to circular RNA, suggesting its potential for RNA therapeutics. Pt1 directly recruits canonical poly(A) polymerases (PAPγ/α)—previously thought to function exclusively in transcription-coupled nuclear pre-mRNA processing—to drive cytoplasmic polyadenylation. Together, these findings chart the rich landscape of viral regulation, extend the scope of poly(A)-tail biology, and establish the virome as a valuable source for uncovering host RNA regulatory mechanisms.
HostingRepository	PRIDE
AnnounceDate	2026-06-21
AnnouncementXML	Submission_2026-06-20_20:25:05.696.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chemin Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-25 06:41:48	ID requested
⏵ 1	2026-06-20 20:25:06	announced

Dataset with its publication pending

submitter keyword: None

V. Narry
contact affiliation	School of Biological Sciences, Seoul National University Center for RNA Research, Institute for Basic Science
contact email	narrykim@snu.ac.kr
lab head
Chemin Lee
contact affiliation	School of Biological Sciences, Seoul National University, Seoul 08826, Republic of Korea.
contact email	cmlee1260@snu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD071193

PRIDE project URI

• PRIDE
  1. PXD071193
     1. Label: PRIDE project
     2. Name: Functional atlas of vertebrate viral RNA elements that stabilize RNA and enhance translation