⮝ Full datasets listing
PXD071174
PXD071174 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ultrasensitive Phosphoproteome Analysis of Microscale Samples via Rapid In-Situ One-Tip IMAC Enrichment Strategy |
| Description | In this study, we developed a novel phosphopeptide enrichment tip (Ti-PAN) based on highly hydrophilic textile PAN fibers, enabling rapid in-situ One-Tip IMAC enrichment within 8 minutes. Using this approach, we identified 2,139 to 17,546 phosphosites across 645 to 3,531 phosphoproteins from samples ranging from 100 to 100,000 cells. This high sensitivity enabled comprehensive mapping of phosphosites in microsamples, including the ERK interactome and mouse brain slices. Furthermore, we successfully applied this strategy to analyze the phosphoproteome of mouse oocytes at different developmental stages using as few as 5 oocytes per sample, identifying 2,709 phosphosites on 701 phosphoproteins. To our knowledge, this represents the deepest coverage of phosphoproteomes from such minimal oocyte material reported to date, offering valuable mechanistic insights into developmental controls during oocyte maturation. This Ti-PAN-based method provides a robust, versatile, and automatable platform for large-scale, highly sensitive phosphopeptide enrichment, substantially advancing phosphoproteomic studies in complex biological systems. |
| HostingRepository | iProX |
| AnnounceDate | 2025-11-25 |
| AnnouncementXML | Submission_2025-11-25_01:03:59.296.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ci Wu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-25 00:56:55 | ID requested | |
| ⏵ 1 | 2025-11-25 01:03:59 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Phosphoproteome, Microscale Samples, IMAC Enrichment, Mouse oocytes |
Contact List
| Ci Wu | |
|---|---|
| contact affiliation | Liaoning Normal University |
| contact email | wuci1111@126.com |
| lab head | |
| Ci Wu | |
| contact affiliation | Liaoning Normal University |
| contact email | wuci1111@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
