⮝ Full datasets listing
PXD071168
PXD071168 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Pull-down results of proteins interacting with the alkaline phosphatase G-quadruplex in MC3T3-E1 cells |
| Description | G-quadruplexes (G4s) are non-canonical nucleic acid structures that participate in diverse biological processes, yet their functional involvement in endogenous regulatory events governing osteogenic differentiation remains largely unexplored. In the present study, we observed a progressive decline in global G4 abundance during osteogenesis and demonstrated that small-molecule stabilization of G4 structures markedly impairs osteogenic progression in both cellular and in vivo models. To uncover the molecular basis of this regulatory effect, we employed DNA affinity precipitation combined with liquid chromatography–tandem mass spectrometry (LC-MS/MS) to identify proteins that interact with a G4 structure embedded within the promoter region of the osteogenic master regulator alkaline phosphatase (Alpl). This unbiased proteomic screening revealed multiple candidate G4-binding proteins, among which Nucleolin (NCL) and the G4-resolving helicase Dhx36 were further validated by immunoblotting. Mechanistically, these two proteins exert opposing influences on Alpl transcriptional control: Nucleolin stabilizes the Alpl promoter G4 to repress promoter activity, whereas Dhx36 unwinds the G4 structure, thereby facilitating transcriptional activation. |
| HostingRepository | iProX |
| AnnounceDate | 2025-11-25 |
| AnnouncementXML | Submission_2025-11-24_23:09:33.295.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Fujun Jin |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-24 23:09:15 | ID requested | |
| ⏵ 1 | 2025-11-24 23:09:33 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Alkaline phosphatase G-quadruplex，G-quadruplex binding protein, MC3T3-E1 |
Contact List
| Fujun Jin | |
|---|---|
| contact affiliation | School of Biomedical and Pharmaceutical Sciences |
| contact email | jinfujun@gdut.edu.cn |
| lab head | |
| Fujun Jin | |
| contact affiliation | School of Biomedical and Pharmaceutical Sciences |
| contact email | jinfujun@gdut.edu.c |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
