PXD071165

PXD071165 is an original dataset announced via ProteomeXchange.

Title	Mapping Core Components of Membrane-less Organelles in Living Cells by Phase-APEX2 Proximity Labeling
Description	This project contains the raw proteomic files (.raw) of membraneless organelles (MLOs) obtained using the Phase-APEX2-MS technique. Phase-APEX2-MS is a newly developed, photo-activatable, and highly specific proximity labeling method for targeting MLOs. It enables the proteomic analysis of specific MLOs within living cells in just 120 seconds. The technique shows high specificity, allowing for the capture of core MLO components while significantly minimizing interference from other cellular compartments. The project includes four datasets, corresponding to batches 1 to 4 mentioned in the publication, which cover two types of MLOs: the nucleolus and stress granules. The data acquisition instruments and experimental procedures for each dataset are described in the "Methods" section of the paper.
HostingRepository	iProX
AnnounceDate	2025-11-28
AnnouncementXML	Submission_2026-06-01_19:26:49.837.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhuo Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; Q Exactive Plus; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-24 22:53:18	ID requested
⏵ 1	2026-06-01 19:26:50	announced

Dataset with its publication pending

submitter keyword: Membrane-less organelles, phase separation, nucleolus, stress granule,proximity labeling

Pilong Li
contact affiliation	Tsinghua University
contact email	pilongli@mail.tsinghua.edu.cn
lab head
Zhuo Chen
contact affiliation	Tsinghua University
contact email	791863987@qq.com
dataset submitter

iProX dataset URI