⮝ Full datasets listing
PXD071147
PXD071147 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Global proteomics and ubiquitomics in THP-1 derived macrophages stably expressing SspH2 WT/C580A |
| Description | THP1 cells stably expressing WT or catalytic mutant C580A SspH2 were differentiated into macrophages, and treated with MG-132 to enrich ubiquitinated proteins. Cells were lysed with probe sonicator in 9 M urea, and proteins were reduced, alkylated, digested with trypsin, and desalted before diGly-modified peptides were isolated using the PTMScan HS Ubiquitin/SUMO Remnant Motif Kit (Cell Signaling Technology, cat. no. 59322) on a KingFisher Duo Prime system. Enriched (Glygly; GG) and flow-through peptide (total peptides; TP) fractions were collected. The enriched GG fractions were run with both data-dependent (DDA) and data-independent acquisition (DIA) set at 36 m/z isolation window widths, and analyzed on Spectronaut with DDA/DIA hybrid extension analysis for GG site identification, and the TP fractions were only run with DIA. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-24 |
| AnnouncementXML | Submission_2025-11-24_15:35:32.981.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Olivier Julien |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Acetyl; ubiquitination signature dipeptidyl lysine; Oxidation |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-24 14:24:23 | ID requested | |
| ⏵ 1 | 2025-11-24 15:35:33 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: SspH2, Ubiquitomics, Salmonella, GlyGly, DatasetType:Proteomics |
Contact List
| Olivier Julien | |
|---|---|
| contact affiliation | University of Alberta |
| contact email | ojulien@ualberta.ca |
| lab head | |
| Amit Bhavsar | |
| contact affiliation | University of Alberta |
| contact email | apbhavsa@ualberta.ca |
| lab head | |
| Olivier Julien | |
| contact affiliation | University of Alberta |
| contact email | ojulien@ualberta.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100008/ |
