PXD071144

PXD071144 is an original dataset announced via ProteomeXchange.

Title	A multidisciplinary integrated approach for the identification and characterization of the AMP profile in Hermetia illucens hemolymph
Description	The growing threat of antimicrobial resistance calls for alternative strategies to conventional antibiotics. Insects represent a promising source of antimicrobial peptides (AMPs) due to their potent innate immune responses. In this study, we investigated the peptide extracts from hemolymph of Hermetia illucens larvae as a bioresource of infection-induced AMPs. Larvae were challenged with Gram-negative (Escherichia coli) and Gram-positive (Micrococcus flavus) bacteria, and hemolymph-derived peptides were extracted and fractionated via reverse-phase high-performance liquid chromatography (RP-HPLC). Antibacterial activity of peptide fractions was evaluated in vitro against a panel of pathogenic strains, including multidrug-resistance isolated such as carbapenem-resistant Klebsiella pneumoniae. The peptide fractions were further analyzed by SDS-PAGE and LC-MS/MS to identify candidate AMPs. In parallel, shotgun proteomics on unfractionated peptide extracts enabled quantitative comparison across experimental conditions. In silico analysis using ProP 1.0, APD3, and CAMPR4 prediction tools revealed physicochemical profiles and machine learning-based scores consistent with antimicrobial function. Our integrative approach confirms that bacterial infection induces a diverse and bioactive immunopeptidome in H. illucens, supporting its potential as a sustainable platform for discovery of novel antimicrobial agents.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-05-31_16:27:37.718.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ilaria iacobucci
SpeciesList	scientific name: Hermetia illucens; NCBI TaxID: NEWT:343691;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-24 13:21:09	ID requested
⏵ 1	2026-05-31 16:27:38	announced

De Stefano F, Monaco V, Giglio F, Scieuzo C, Rinaldi R, Salvia R, Quaranta G, Amaro S, Fusco A, Mancini IM, Monti M, Donnarumma G, Falabella P, Hemolymph. Insects, 17(5):(2026) [pubmed]

10.3390/insects17050486;

submitter keyword: Antimicrobial peptides

Hermetia illucens

Antimicrobial resistance

Proteomics

In vitro antibacterial assay

In silico prediction

Maria Monti
contact affiliation	University of Naples Federico II and CEINGE Biotecnologie Avanzate Franco Salvatore
contact email	montimar@unina.it
lab head
ilaria iacobucci
contact affiliation	University of Naples Federico II
contact email	ilaria.iacobucci@unina.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071144
     1. Label: PRIDE project
     2. Name: A multidisciplinary integrated approach for the identification and characterization of the AMP profile in Hermetia illucens hemolymph