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PXD071077

PXD071077 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphorylation site identifcaiton of OsALDH2B1
DescriptionTo identify the residues of OsALDH2B1 phosphorylated by OsSERL1, the in vitro kinase assays were conducted by incubating recombinant MBP-OsSERL1 + MBP-OsALDH2B1 and MBP + MBP-OsALDH2B1. The reaction was terminated by adding SDS loading buffer to a 1×concentration, and the reaction products were subjected to CBB staining and immunoblot analysis as described above. The CBB-stained protein bands corresponding to phosphorylated OsHSFA4d were excised and subjected to LC-MS/MS analysis.
HostingRepositoryPRIDE
AnnounceDate2026-08-24
AnnouncementXMLSubmission_2026-08-23_16:13:20.975.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitteryinggeng ke
SpeciesList scientific name: Oryza sativa (Rice); NCBI TaxID: NEWT:4530;
ModificationListO-phosphopantetheine-L-serine
InstrumentOrbitrap Astral
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-11-22 15:36:22ID requested
12026-08-23 16:13:23announced
Publication List
10.1111/jipb.70210;
Ma Z, Gao X, An S, Chen M, Zhang B, Zhou L, Sun S, Li M, Yu F, L, ü S, Chu Z, You A, Ke Y, Yang P, A leucine-rich-repeat receptor-like kinase SERL1 phosphorylates and stabilizes OsALDH2B1 to promote alkaline tolerance and grain size in rice. J Integr Plant Biol, 68(7):2037-2053(2026) [pubmed]
Keyword List
submitter keyword: phosphorylation site identification,Phosphorylation,protein
Contact List
Yinggen Ke
contact affiliationSchool of Life Sciences, Hubei University, Wuhan 430062, China
contact emailygke@hubu.edu.cn
lab head
yinggeng ke
contact affiliationSchool of Life Sciences, Hubei University, Wuhan, China
contact emailygke@hubu.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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