PXD071023 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evaluating the performance of photon- and electron-based fragmentation methods in Omnitrap-LCMS analysis of N-glycopeptides |
| Description | To date, collision-induced dissociation and methods based on electron transfer dissociation are considered the standard approaches for the mass spectrometry analysis of N- and O-glycopeptides, respectively, allowing for identification of both peptide and glycan compositions. In recent years, alternative fragmentation methods such as ultraviolet photodissociation (UVPD) and more energetic versions of electron-based techniques (such as electron ionisation dissociation, EID) have been shown to be useful for the analysis of glycopeptides, producing rich information on the glycopeptide structure, including types of glycosidic linkages. We performed a series of LCMS (liquid chromatography coupled with mass spectrometry) experiments to assess the efficacy of UVPD, EID, and electron capture dissociation (ECD) for the analysis of complex glycopeptide mixtures, and we discuss challenges associated with automated analysis of glycopeptide LCMS data. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-09 |
| AnnouncementXML | Submission_2026-09-09_02:23:49.540.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Nikita Levin |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-20 17:49:16 | ID requested | |
| ⏵ 1 | 2026-09-09 02:23:51 | announced | |
Publication List
Keyword List
| submitter keyword: glycopeptides, Omnitrap, ECD, UVPD, EID |
Contact List
| Shabaz Mohammed |
|---|
| contact affiliation | The Rosalind Franklin Institute, Harwell Science and Innovation Campus, UK Department of Chemistry, University of Oxford, UK Department of Biochemistry, University of Oxford, UK |
| contact email | shabaz.mohammed@chem.ox.ac.uk |
| lab head | |
| Nikita Levin |
|---|
| contact affiliation | University of Oxford |
| contact email | nikita.levin@rfi.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071023
- Label: PRIDE project
- Name: Evaluating the performance of photon- and electron-based fragmentation methods in Omnitrap-LCMS analysis of N-glycopeptides
