⮝ Full datasets listing
PXD071005
PXD071005 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Acute in vivo proximity labeling for membrane targeted proteomics in neuronal circuits |
| Description | The field of molecular systems neuroscience aims to bridge neuronal circuit activity patterns with changes in protein expression and localization in the context of specific animal behaviors. However, there are limited tools for capturing changes in subcellulary-defined proteomes within neuronal circuits during activity-gated timescales in vivo. Here, we engineered targeted versions of the proximity labeling enzyme TurboID, to tag proteins at the neuronal membrane during a user-delivered biotin injection. We optimized a labeling strategy that enables a one-to-two-hour labeling window to tag proteins in prefrontal cortex (PFC) cell bodies and corresponding axons in two downstream projections. We performed proteomics to identify proteins enriched across different projections, and identified membrane associated proteins in the PFC cell bodies that are enriched at the membrane following an acute cocaine injection. These advancements enable the detection of proteins at the subcellular level within short labeling windows, allowing identification of stimulus-specific proteomes in behaving mice. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-01 |
| AnnouncementXML | Submission_2025-12-01_10:12:40.042.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brett Phinney |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | timsTOF HT; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-20 12:00:17 | ID requested | |
| ⏵ 1 | 2025-12-01 10:12:40 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: proximity labeling, TurboID, subcellular proteomics, medial prefrontal cortex, neuronal circuit projections, cocaine, axonal proteomics, DatasetType:Proteomics |
Contact List
| Christina K. Kim1 | |
|---|---|
| contact affiliation | Princeton |
| contact email | ckk@princeton.edu |
| lab head | |
| Brett Phinney | |
| contact affiliation | UC Davis |
| contact email | brettsp@ucdavis.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099974/ |
