PXD070997

PXD070997 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of Control and Palbociclib-treated HeLa cells
Description	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to profile the proteomes of Control and Palbociclib-treated HeLa cells. For protein identification and quantification, mass spectrometry data from fractionated samples (data-dependent acquisition, DDA; 6 fractions) and single-shot samples (data-independent acquisition, DIA) were utilized to construct a DDA library and a direct-DIA library, respectively. These libraries were computationally merged into a hybrid spectral library using Spectronaut software (Biognosys). Subsequent functional annotation was performed through Gene Ontology (GO) and InterPro (IPR) analyses using the InterProScan-5 tool against the non-redundant protein database. Additionally, protein family and pathway analyses were carried out using the Clusters of Orthologous Groups (COG) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases.
HostingRepository	PRIDE
AnnounceDate	2025-12-04
AnnouncementXML	Submission_2025-12-04_08:02:16.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yihang Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-20 09:26:17	ID requested
⏵ 1	2025-12-04 08:02:16	announced

Dataset with its publication pending

submitter keyword: Palbociclib，HeLa cells，LC-MS/MS

Lijun Liu
contact affiliation	Institute of Biotechnology, College of Life and Health Sciences, Northeastern University, Shenyang, China
contact email	liulijun@mail.neu.edu.cn
lab head
Yihang Wang
contact affiliation	Northeastern university
contact email	1420899467@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070997

PRIDE project URI

• PRIDE
  1. PXD070997
     1. Label: PRIDE project
     2. Name: Proteomics analysis of Control and Palbociclib-treated HeLa cells