PXD070961

PXD070961 is an original dataset announced via ProteomeXchange.

Title	Generation of human iPSC-derived macrophages under animal-free conditions enables their application for safety and efficacy testing
Description	Generation and use of human macrophages in vitro are essential for a variety of (therapeutic) applications. Recently, there has been growing interest in macrophages derived from induced pluripotent stem cells (iPSC-Mac), which has led to the development of numerous differentiation protocols. These protocols typically involve a stepwise differentiation process using well-defined culture media, though the final differentiation stage often relies on undefined animal serum components, such as fetal bovine serum (FBS). In this study, we assessed the impact of a serum-containing medium in comparison to a fully defined medium (X-VIVO 15TM) on human iPSC-Mac function. While both media compositions effectively produced fully functional human macrophages, we observed significant differences in their stage of activation and their functional responsiveness. Macrophages differentiated under the defined, serum-free conditions showed a more neutral activation state, an enhanced cholesterol metabolism, and were more sensitive to post pro- or anti-inflammatory stimulation compared to serum-grown cells. Most important, iPSC-Mac from these cultures also exhibit a more robust and reproducible response to co-stimulatory signals with IFNγ or lipopolysaccharide (LPS) in combination with the immune-suppressive agent dexamethasone. In contrast, serum-grown iPSC-Mac demonstrated a more pre-activated state, with noteworthy background levels of interleukin 6 (IL-6). Upon stimulation, these cells showed reduced sensitivity and responsiveness towards secondary signals, underscoring potential challenges when using FBS-based media for drug screening or immunomodulatory assessments. Given the enhanced responsiveness to co-stimulatory signals makes iPSC-Mac from defined-cultures more suitable for testing immunomodulatory drugs, novel bio-assays and in vivo applications.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_19:08:44.790.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lothar Jaensch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-19 14:53:15	ID requested
⏵ 1	2026-04-26 19:08:45	announced

10.1186/s13287-026-05009-1;

Schinke M, Gensch I, Rietschel T, Nguyen AHH, Slevogt H, J, ä, nsch L, Lachmann N, Defined culture conditions improve functional properties of mature iPSC-derived macrophages for therapeutic screening. Stem Cell Res Ther, 17(1):(2026) [pubmed]

submitter keyword: Macrophages, Drug screening, Physiologic media,hiPSC, Cell manufacturing, Surfaceome, cell surface proteomics, RNA-Seq

Lothar Jänsch
contact affiliation	Cellular Proteome Research, Helmholtz-Centre for Infection Research, Braunschweig, Germany
contact email	Lothar.Jaensch@helmholtz-hzi.de
lab head
Lothar Jaensch
contact affiliation	HZI
contact email	lothar.jaensch@helmholtz-hzi.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD070961

PRIDE project URI

• PRIDE
  1. PXD070961
     1. Label: PRIDE project
     2. Name: Generation of human iPSC-derived macrophages under animal-free conditions enables their application for safety and efficacy testing