PXD070947

PXD070947 is an original dataset announced via ProteomeXchange.

Title	Vitamin B6 form produced by Lactobacillus induces metabolic disorder and suppresses multi-pathogenic bacteria
Description	Vitamin B6 comprises six vitamers, pyridoxal, pyridoxine, pyridoxamine, pyridoxal 5′-phosphate (PLP), pyridoxine 5′-phosphate (PNP), and pyridoxamine 5′-phosphate (PMP), recognized for pleiotropic functions in mitigating oxidative stress and modulating metabolic homeostasis. This study reveals that PMP exhibits broad-spectrum antibacterial activity against pathogens, including Aeromonas hydrophila (A. hydrophila), Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella. In vitro assays demonstrated that high-dose PMP disrupts bacterial membrane integrity, triggering extensive extracellular DNA leakage and bactericidal effects. Furthermore, we identified Pediococcus acidilactici GR-6 and Lactobacillus fermentum GR-7, isolated from crucian carp gut, that synergistically synthesize vitamin B6 de novo during pathogens co-culture, achieving ≥63% inhibition of polymicrobial pathogens. Integrated genomic, proteomic and metabolomic analyses confirm that GR-6/GR-7 consortium regulates pyridoxal kinase and pyridoxine 4-dehydrogenase, maintaining vitamin B6 primarily as pyridoxine (84%) and PMP (16%). In A. hydrophila-infected crucian carp, dietary supplementation with GR-6/GR-7 consortium increases survival by 50%, with restored gut microbiota diversity and attenuated systemic inflammation (Alkaline phosphatase, Lysozyme and GSH/GSSG ratio). Metabolomics showed that probiotics-mediated elevation of intestinal content PMP level directly inhibits A. hydrophila infection. Collectively, this study establishes PMP as a novel probiotic metabolite that directly eliminates pathogens and resolving gut microbiome-host metabolic dysregulation.
HostingRepository	PRIDE
AnnounceDate	2026-01-26
AnnouncementXML	Submission_2026-01-25_16:22:29.703.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ying Wu
SpeciesList	scientific name: Carassius; NCBI TaxID: NEWT:7956;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-19 08:50:24	ID requested
⏵ 1	2026-01-25 16:22:30	announced

Wu Y, Ling Z, Peng L, Wang X, He Y, Khan A, Salama ES, Kulshreshtha S, Liu P, Li X, form produced by Lactobacillus induces metabolic disorder and suppresses multi-pathogenic bacteria. Commun Biol, 9(1):82(2025) [pubmed]

10.1038/s42003-025-09348-2;

submitter keyword: Probiotic consortium, Pyridoxamine 5'-phosphate, Metabolism, Pathogens,Vitamin B6, Gut microbiome

Ying Wu
contact affiliation	Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou, China
contact email	ywu18@lzu.edu.cn
lab head
Ying Wu
contact affiliation	Life sciences
contact email	ywu18@lzu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070947
     1. Label: PRIDE project
     2. Name: Vitamin B6 form produced by Lactobacillus induces metabolic disorder and suppresses multi-pathogenic bacteria