⮝ Full datasets listing
PXD070937
PXD070937 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | MR-SP2: A Microreactor for Upward Pressure catapulting Laser Microdissection for Mass Spectrometry-Based Spatial Proteomics at Single Cell Resolution |
| Description | This dataset contains LC-MS/MS data acquired to evaluate MR-SP2, a microreactor-based, one-pot sample preparation workflow designed for spatial proteomics of FFPE tissue after laser capture microdissection (LCM). FFPE murine kidney tissue was microdissected using an upward pressure-catapulting LCM system, and defined tissue microregions of 50,000 um^3 (~22 cells), 12,500 um^3 (~5-6 cells), and 3,125 um^3 (~1-2 cells) were isolated. Each LCM-captured specimen was processed either with the MR-SP2 workflow or with a conventional tube-based protocol. The dataset includes raw LC-MS/MS files, sample metadata, and information on specimen size, preparation method, and capture success. The data document improved performance of MR-SP2 across all input levels, including higher capture success rates and increased protein identification depth, especially for few-cell samples. This dataset supports benchmarking of microreacto-based sample preparation for low-input FFPE spatial proteomics and provides reference data for comparing microreactor versus tube-based processing strategies. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-11 |
| AnnouncementXML | Submission_2026-06-11_07:05:12.015.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Maximilian Maldacker |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | CarbamidomethylDTT |
| Instrument | timsTOF fleX |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-19 04:58:33 | ID requested | |
| ⏵ 1 | 2026-06-11 07:05:13 | announced |
Publication List
| Metzger M, Maldacker M, Hutzenlaub T, Paust N, Schilling O, Klatt JN, : A Microreactor-Based Workflow for Few-Cell Spatial Proteomics on the Legacy Zeiss PALM MicroBeam. J Proteome Res, 25(5):2486-2494(2026) [pubmed] |
Keyword List
| submitter keyword: Spatial Proteomics, Sample Preparation, Lasermicrodissection, Few-Cell Proteomics, FFPE, DIA, DatasetType:Proteomics |
Contact List
| Prof. Dr. Oliver Schilling | |
|---|---|
| contact affiliation | Medical Center - University of Freiburg |
| contact email | oliver.schilling@uniklinik-freiburg.de |
| lab head | |
| Dr. Jan-Niklas Klatt | |
| contact affiliation | Hahn-Schickard |
| contact email | niklas.klatt@hahn-schickard.de |
| lab head | |
| Maximilian Maldacker | |
| contact affiliation | Institute for Surgical Pathology, Medical Center Freiburg |
| contact email | maximilian.maldacker@uniklinik-freiburg.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099955/ |
