⮝ Full datasets listing
PXD070935
PXD070935 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Photocatalytic Eosin Y mediated single electron transfer for spatially resolved proteomics of amyloid aggregates in Alzheimer’s disease |
| Description | Alzheimer’s disease (AD) is characterized by the accumulation of pathological amyloid aggregates, yet methods for spatially resolved, proteome-wide profiling of amyloid composition and their microenvironment remain limited. Here, we report Eosin Y (EY) as an easily accessible and efficient organic photocatalyst for pan-amyloid binding, labeling, and proteomic profiling in AD brain tissues. EY demonstrated strong binding affinity toward various amyloids. Upon green-light irradiation in the presence of aryl azide, EY enabled rapid and covalent labeling of amyloid structures and this process was demonstrated to be a single-electron transfer (SET) pathway. Application of this strategy to 5×FAD mouse brain tissue enabled successful enrichment and proteomic identification of key AD related proteins, including App, Apoe, and Gfap. Together, this work demonstrates a microscopy free method for the molecular dissection of amyloid plaques, advancing the toolbox for neurodegenerative disease research |
| HostingRepository | iProX |
| AnnounceDate | 2025-11-19 |
| AnnouncementXML | Submission_2025-11-19_00:00:29.781.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Fangliang Guo |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-19 00:00:07 | ID requested | |
| ⏵ 1 | 2025-11-19 00:00:30 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: amyloid, Photocatalyst, Alzheimer’s disease |
Contact List
| Yu Liu | |
|---|---|
| contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
| contact email | liuyu@dicp.ac.cn |
| lab head | |
| Fangliang Guo | |
| contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
| contact email | 1097157451@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
