PXD070916

PXD070916 is an original dataset announced via ProteomeXchange.

Title	Crotoxin B from the South American Rattlesnake Crotalus vegrandis Blocks Voltage-Gated Calcium Channels Independent of Its Intrinsic Catalytic Activity
Description	Neurotoxicity following South American Crotalus rattlesnake bite is primarily caused by crotoxin, the most abundant component in their venom. Despite the central role of volt-age-gated calcium channels (CaV) in neurotransmission, direct targetability by crotoxin has been poorly explored. Crotoxin is a non-covalent heterodimer formed by an acidic subunit (CA) and a basic toxic phospholipase A2 subunit (CB). Here, we chromatograph-ically isolated the CB subunit from Crotalus vegrandis and studied its effect on CaV heter-ologously expressed in HEK293 cells using whole-cell patch-clamp technique. Mass spectrometry analysis identified a protein that matched with 97% sequence coverage the CBc isoform from Crotalus durissus terrificus. Isolated CB exhibited moderate phospholipase activity that was not correlated to its cytotoxic effect on cultured tsA201 cells. Using Ba2+ as charge carrier to prevent the enzymatic activity, we found that CB inhibited currents me-diated by the N-type CaV2.2 and CaV1.2 L-type calcium channels, in a dose-dependent manner, with higher potency for the latter, and negligible changes in the voltage depend-ence of channel activation. Our results reveal a novel phospholipase-independent biolog-ical activity and a novel molecular target of CB providing new insights into the patho-physiology of Crotalus snakebite envenoming with potential clinical therapeutic implica-tions
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:27:02.056.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Beatrix Santiago-Schuebel
SpeciesList	scientific name: Crotalus vegrandis; NCBI TaxID: NEWT:184545;
ModificationList	iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-18 15:55:33	ID requested
⏵ 1	2026-02-01 16:27:03	announced

10.3390/toxins18010036;

Eicheldinger M, Miranda-Laferte E, Castilla F, Jordan N, Santiago-Sch, ü, bel B, Hidalgo P, Blocks Voltage-Gated Calcium Channels Independent of Its Intrinsic Catalytic Activity. Toxins (Basel), 18(1):(2026) [pubmed]

submitter keyword: None

Dr. Beatrix
contact affiliation	Forschungszentrum Jülich GmbH Institute of Biological Information Processing Structural Biochemistry (IBI-7)
contact email	b.santiago-schuebel@fz-juelich.de
lab head
Beatrix Santiago-Schuebel
contact affiliation	Forschungszentrum Juelich, ZEA-3
contact email	b.santiago-schuebel@fz-juelich.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070916
     1. Label: PRIDE project
     2. Name: Crotoxin B from the South American Rattlesnake Crotalus vegrandis Blocks Voltage-Gated Calcium Channels Independent of Its Intrinsic Catalytic Activity