PXD070911

PXD070911 is an original dataset announced via ProteomeXchange.

Title	α7 Nicotinic Acetylcholine Receptor-Associated Signaling in U87MG Glioblastoma Cells (LFQ, Orbitrap Elite)
Description	This project investigates the role of α7 nicotinic acetylcholine receptor (α7 nAChR) signaling in glioblastoma using the U87MG human glioblastoma cell line. Cells were treated with selective α7 nAChR ligands (10 μM of agonist PNU282987, 1 μM of positive allosteric modulator PNU120596, 1 μM of antagonist α-cobratoxin) and their combination. Whole-cell lysates were processed for total proteome analysis, proteins were digested in-solution with trypsin and peptides analyzed by nano-ESI-LC-MS/MS on a Thermo Orbitrap Elite using DDA. Data were searched with PEAKS Studio 11 against the UniProtKB Human database and quantified by label-free quantification (LFQ). Proteomic changes in cytoskeletal, metabolic and ER-associated proteins were detected.
HostingRepository	PRIDE
AnnounceDate	2026-06-09
AnnouncementXML	Submission_2026-06-08_17:04:05.801.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD070911
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Elena Gondarenko
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-18 14:36:41	ID requested
⏵ 1	2026-06-08 17:04:06	announced

10.6019/PXD070911;

10.1007/s12031-026-02523-y;

submitter keyword: glioblastoma

U87MG

α7 nicotinic acetylcholine receptor

choline

total proteome

label-free quantification

Orbitrap Elite

proteomics

Igor Kasheverov
contact affiliation	Laboratory of Ligand-Receptor Interactions, Department of molecular neuroimmun signaling, Shemyakin-Ovchinnikov Institute of bioorganic chemistry of Russian academy of sciences, Moscow, Russian Federation
contact email	shak_ever@yahoo.com
lab head
Elena Gondarenko
contact affiliation	IBCH RAS
contact email	gondarenkoea@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD070911

PRIDE project URI

• PRIDE
  1. PXD070911
     1. Label: PRIDE project
     2. Name: α7 Nicotinic Acetylcholine Receptor-Associated Signaling in U87MG Glioblastoma Cells (LFQ, Orbitrap Elite)