PXD070903

PXD070903 is an original dataset announced via ProteomeXchange.

Title	AR RIME in Human Prostate Cancer Cells
Description	BPTF, the scaffolding subunit of the nucleosome remodeling factor (NURF) complex, has been implicated in the progression of several malignancies, but its role in prostate cancer (PCa) remains unclear. Here, we demonstrate that BPTF is upregulated in castration-resistant prostate cancer (CRPC) and promotes disease progression. RNA-seq revealed that BPTF primarily enhances the expression of androgen receptor (AR) target genes. ChIP-seq showed that BPTF increases AR binding at promoters, enhancers and super-enhancers. ATAC-seq further demonstrated that BPTF increases chromatin accessibility to facilitate AR binding, in part through SMARCA1, a catalytic subunit of the NURF complex. Notably, BPTF/AR co-bound regions are highly enriched for FOXA1 motifs but only weakly enriched for AR motifs. We further show that BPTF forms a protein complex with AR and FOXA1, in which FOXA1 recruits the BPTF-AR complex to chromatin, while BPTF stabilizes the AR-FOXA1 interaction. Importantly, BPTF interacts with AR through its bromodomain, and a BPTF bromodomain inhibitor disrupts this interaction, impairs AR signaling and suppresses PCa cell growth. In summary, our findings establish BPTF as a critical regulator of AR activity by promoting chromatin accessibility and stabilizing the AR-FOXA1 complex, highlighting BPTF as a potential therapeutic target in prostate cancer.
HostingRepository	PRIDE
AnnounceDate	2025-11-19
AnnouncementXML	Submission_2025-11-18_16:08:05.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianfei Qi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-18 12:45:27	ID requested
⏵ 1	2025-11-18 16:08:06	announced

Dataset with its publication pending

submitter keyword: AR,Human prostate cancer cell, Rv1

Jianfei Qi
contact affiliation	Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, MD 21201. Marlene and Stewart Greenebaum Comprehensive Cancer Center, Baltimore, MD 21201.
contact email	jqi@som.umaryland.edu
lab head
Jianfei Qi
contact affiliation	University of Maryland Baltimore
contact email	jqi@som.umaryland.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070903
     1. Label: PRIDE project
     2. Name: AR RIME in Human Prostate Cancer Cells