PXD070901

PXD070901 is an original dataset announced via ProteomeXchange.

Title	Biocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells
Description	Fluorescent probes enable the visualization of dynamic cellular processes with high precision, particularly when coupled with super-resolution imaging techniques that surpass the diffraction limit. Traditional methods include fluorescent protein fusion (e.g., GFP) or organic fluorophores linked to ligands targeting the protein of interest. However, these approaches often introduce functional disruptions or ligand-associated biological effects. Herein, we address these challenges by developing covalent fluorescent probes for endogenous tubulin, a critical cytoskeletal protein involved in processes such as cell movement, division, and biomolecule trafficking. Using well-known tubulin binder cabazitaxel and cell permeable fluorophore silicon-rhodamine as a basis, we introduce a novel biocompatible cleavable linker containing a sulfonium center. This allowed the construction of the optimized probe, 6-SiR-o-C9-CTX, demonstrating excellent cell permeability, fluorogenic properties, and the ability to covalently label tubulin across various human cell lines. Importantly, the targeting moiety could be washed out while preserving tubulin staining, ensuring minimal disruption of tubulin function. This labeling technique was verified by mass spectrometry and it is compatible with STED nanoscopy in both live and fixed cells, offering a powerful high-resolution imaging tool.
HostingRepository	MassIVE
AnnounceDate	2025-11-19
AnnouncementXML	Submission_2025-11-19_07:33:31.357.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olexandr Dybkov
SpeciesList	scientific name: Sus scrofa; common name: pig; NCBI TaxID: 9823;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-18 11:46:33	ID requested
⏵ 1	2025-11-19 07:33:32	announced

Lukinavi, č, ius G, Auvray M, Koenen T, Dybkov O, Urlaub H, Biocompatible Sulfonium-Based Covalent Probes For Endogenous Tubulin Fluorescence Nanoscopy In Live And Fixed Cells. Res Sq, ():(2025) [pubmed]

submitter keyword: 6-SiR-o-C9-CTX, fluorescent probes, in vivo, STED nanoscopy, DatasetType:Proteomics

Prof. Henning Urlaub
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	henning.urlaub@mpinat.mpg.de
lab head
Dr. Grazvydas Lukinavicius
contact affiliation	Max Planck Institute for Multidisciplinary Sciences
contact email	grazvydas.lukinavicius@mpinat.mpg.de
lab head
Olexandr Dybkov
contact affiliation	MPINAT
contact email	odybkov@mpinat.mpg.de
dataset submitter

MassIVE dataset URI

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