⮝ Full datasets listing
PXD070895
PXD070895 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Chlamydial membrane vesicles deliver the beta barrel membrane protein OmpA to mitochondria to inhibit apoptosis |
| Description | Chlamydiae are obligate intracellular bacteria that inhibit mitochondrial apoptosis to maintain integrity of the host cell. We have previously reported that a chlamydial outer membrane b-barrel protein, OmpA, can during ectopic expression inhibit mitochondrial apoptosis through direct interaction with the BCL-2-family effectors BAX and BAK. We here show that OmpA from Chlamydia trachomatis (Ctr) uses membrane vesicles for its delivery to the outer mitochondrial membrane during Ctr infection. Using a number of imaging and fractionation techniques, we show that OmpA during infection reaches mitochondria and is inserted into mitochondrial membranes. Chlamydia derived vesicles (CDV) from Ctr-infected cells contained OmpA as well as other outer membrane proteins and LPS. When added to uninfected cells, CDVs fused with mitochondrial membranes, causing the interaction of OmpA with BAK and the cytosolic retro-translocation of BAX. CDV addition to uninfected cells also protected the cells against apoptosis. We previously showed tha OmpA works in co-ordination with VDAC2 to block apoptosis and here propose a structural model of this BAK inhibition by OmpA that reenacts the inhibition of BAK by VDAC2. The results provide evidence that OmpA from Chlamydia, as well as the structurally similar ortholog from the related Simkania, specifically exploits its relationship to mitochondrial porins to protect the infected cell against apoptosis and to enable intracellular growth of the bacteria in human cells. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-26 |
| AnnouncementXML | Submission_2026-01-26_09:22:43.864.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Stefan Tholen |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Chlamydia trachomatis; NCBI TaxID: 813; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF fleX |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-18 09:03:58 | ID requested | |
| ⏵ 1 | 2026-01-26 09:22:44 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Chlamydia, Apoptosis inhibition, MOMP, OmpA, Mitochondria, Vesicles, Outer membrane, DatasetType:Proteomics |
Contact List
| Prof. Dr. Georg Haecker | |
|---|---|
| contact affiliation | Center for Microbiology and Hygiene Freiburg |
| contact email | georg.haecker@uniklinik-freiburg.de |
| lab head | |
| Stefan Tholen | |
| contact affiliation | Uniklinik Freiburg |
| contact email | stefan.tholen@uniklinik-freiburg.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099942/ |
