PXD070842

PXD070842 is an original dataset announced via ProteomeXchange.

Title	High-resolution spatial proteomics of Plasmodium falciparum schizonts using hyperLOPIT and TMT-based mass spectrometry
Description	This dataset contains a hyperLOPIT spatial proteomics experiment performed on Plasmodium falciparum to determine the subcellular localization of parasite proteins. Schizont-stage parasites were fractionated by density gradient centrifugation, and collected fractions were labelled with tandem mass tags (TMT) for multiplexed quantification. Peptides were analysed by LC–MS/MS on an Orbitrap Fusion Lumos mass spectrometer. Protein identification and quantification were performed using Proteome Discoverer with the Sequest HT search engine against the P. falciparum reference proteome and human proteome. The dataset supports high-resolution mapping of organelle localization and contributes to understanding the compartmental organization of the malaria parasite proteome.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-06-01_05:35:35.219.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD070842
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Scott Chisholm
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: NEWT:36329;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-17 08:24:55	ID requested
⏵ 1	2026-06-01 05:35:35	announced

10.6019/PXD070842;

Chisholm SA, Flores V, Kemp A, Breckels LM, Koreny L, Dos Santos Pacheco N, Barylyuk K, Kuroshchenkova A, Lilley KS, Rayner JC, Waller RF, The spatial proteome of the Plasmodium falciparum schizont illuminates the composition and evolutionary trajectories of its organelles. Nat Commun, ():(2026) [pubmed]

10.1038/s41467-026-73664-2;

submitter keyword: None

Ross Waller
contact affiliation	Department of Biochemistry, University of Cambridge, United Kingdom
contact email	rfw26@cam.ac.uk
lab head
Scott Chisholm
contact affiliation	Department of Biochemistry, University of Cambridge
contact email	sc2203@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD070842

PRIDE project URI

• PRIDE
  1. PXD070842
     1. Label: PRIDE project
     2. Name: High-resolution spatial proteomics of Plasmodium falciparum schizonts using hyperLOPIT and TMT-based mass spectrometry