PXD070785

PXD070785 is an original dataset announced via ProteomeXchange.

Title	Neutrophil secretory proteins inhibit calcium oxalate crystallization and crystal growth, but promote crystal aggregation
Description	Neutrophil secretory proteins are frequently found in calcium oxalate (CaOx) kidney stone matrix, suggesting their involvement in stone pathogenesis, but with unclear mechanisms. We therefore investigated the effects of secretome (a set of secretory proteins) from CaOx monohydrate (COM)-exposed vs. control neutrophils on crystal nucleation (crystallization), growth, aggregation and invasion. Quantitative proteomics was also performed to identify significantly altered secretory proteins, followed by analyses of their physicochemical properties and biological relevance. The data demonstrated that both COM-treated and control secretomes inhibited crystallization and crystal growth, but the inhibitory effects from the COM-treated secretome were slightly weaker. By contrast, both of them promoted crystal aggregation, with the more potent effect from the COM-treated secretome. However, neither of them had a modulatory effect on crystal invasion. Quantitative proteomics revealed 20 decreased and 9 increased proteins in the COM-treated secretome compared with the control. Analyses of physicochemical properties showed that the increased secretory proteins tended to have lower instability index and smaller number of oxalate-binding motifs/protein. Main molecular functions of the increased group were catalytic, hydrolase and transporter activities, whereas those of the decreased group included RNA binding, molecular adaptor activity and catalytic activity. These data indicate that neutrophil secretome inhibits crystallization and crystal growth but promotes crystal aggregation. The COM-treated secretome exerts weaker inhibitory effects on crystallization and growth but has a stronger promoting effect on crystal aggregation. These findings enhance our understanding of the roles of neutrophils in kidney stone pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:30:16.275.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD070785
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Visith Thongboonkerd
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-15 20:03:11	ID requested
⏵ 1	2026-09-07 04:30:17	announced

10.1111/imm.70153;

10.6019/PXD070785;

Lertprapai C, Peerapen P, Thongboonkerd V, Neutrophil Secretory Proteins Inhibit Calcium Oxalate Crystallisation and Crystal Growth, but Promote Crystal Aggregation. Immunology, 179(1):101-117(2026) [pubmed]

submitter keyword: Immune

Kidney stone

Modulators

Proteomics

Secretome

Stone modulation

Prof. Visith Thongboonkerd
contact affiliation	Medical Proteomics Unit, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
contact email	vthongbo@yahoo.com
lab head
Visith Thongboonkerd
contact affiliation	Mahidol University
contact email	sirirajms@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070785
     1. Label: PRIDE project
     2. Name: Neutrophil secretory proteins inhibit calcium oxalate crystallization and crystal growth, but promote crystal aggregation