PXD070784

PXD070784 is an original dataset announced via ProteomeXchange.

Title	Native long-read RNA sequencing of human monocytes reveals activation-induced alternative splicing toward functional isoforms
Description	Alternative splicing is a key mechanism for expanding transcriptomic and proteomic complexity, yet its role in innate immune activation remains incompletely understood. Here, we applied Oxford Nanopore native RNA-sequencing to generate an isoform-level transcriptome of primary human monocytes before and after activation with lipopolysaccharide. We identify over 24,000 expressed isoforms, including thousands of previously unannotated variants. Activation induced widespread isoform-specific expression changes, leading to extensive isoform switching events, validated using matched short-read RNA-Seq. These activation-induced shifts enhanced transcript immune-regulatory functions: activated monocytes preferentially express longer, coding-competent isoforms with complete open reading frames, fewer retained introns, and increased domain complexity. By integrating matched Ribo-seq and proteomic data, we demonstrate that these isoform modulations are associated with enhanced translation of immune effector proteins. Together, our findings position alternative splicing as a dynamic and functional regulator of monocyte activation, emphasizing the need for isoform-level resolution to fully understand immune cell function and inflammation.
HostingRepository	PRIDE
AnnounceDate	2026-04-29
AnnouncementXML	Submission_2026-04-29_09:11:49.172.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harmjan Vos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-15 19:40:15	ID requested
⏵ 1	2026-04-29 09:11:49	announced

Dataset with its publication pending

submitter keyword: monocytes activation-induced alternative splicing isoforms

Jorg van Loosdregt
contact affiliation	Center for Translational Immunology, University Medical Center Utrecht and Utrecht University, Utrecht, the Netherlands
contact email	j.vanloosdregt@umcutrecht.nl
lab head
Harmjan Vos
contact affiliation	University Medical Center Utrecht Center for Molecular Medicine
contact email	h.r.vos-3@umcutrecht.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070784
     1. Label: PRIDE project
     2. Name: Native long-read RNA sequencing of human monocytes reveals activation-induced alternative splicing toward functional isoforms