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PXD070768

PXD070768 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleS. elongatus PCC 7942 Proteome Integral Solubility Alteration (PISA) Proteomics - Light vs Dark with Dense and Dilute
DescriptionPISA experimental assays investigating structural alterations in Light vs Dark growth conditions with Dense and Dilute conditions. Samples are representative of Synechococcus elongatus PCC 7942 cell lysates (BTO:0004304). Purpose: Detecting structural and abundance changes in cyanobacterial cultures grown in conditions mimicking environmental perturbations such as dense light, dense dark, dilute light, and dilute dark. Technique: Proteome Integral Solubility Assay (PISA) and Global Proteomics. Other Details: TMT10 Labelled, Not-fractionated, 3 plexes in total (Plex1- PISA Dense Light and Dark, Plex2- PISA Dilute Light and Dark, Plex3- Global Dilute Light and Dark, No global proteomics for dense samples). Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.
HostingRepositoryMassIVE
AnnounceDate2025-11-19
AnnouncementXMLSubmission_2025-11-19_10:11:49.703.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew Monroe
SpeciesList scientific name: Synechococcus elongatus PCC 7942; NCBI TaxID: 1140;
ModificationListOxidation; TMT6plex
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-11-14 18:23:09ID requested
12025-11-19 10:11:50announced
Publication List
no publication
Keyword List
submitter keyword: Proteome Integral Solubility Alteration, PISA, DatasetType:Proteomics
Contact List
Snigdha Sarkar
contact affiliationPacific Northwest National Laboratory
contact emailsnigdha.sarkar@pnnl.gov
lab head
John T. Melchior
contact affiliationPacific Northwest National Laboratory
contact emailjohn.melchior@pnnl.gov
lab head
Matthew Monroe
contact affiliationPacific Northwest National Laboratory
contact emailmatthew.monroe@pnnl.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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