⮝ Full datasets listing
PXD070732
PXD070732 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In vivo protein N-terminal sequence analysis of WAGO-1 and WAGO-3 in C. elegans |
| Description | A mass spectrometry-based label-free proteomics analysis was performed to study the N-terminal protein sequences of WAGO-1 and WAGO-3 in C. elegans. Argonaute-enriched proteins were digested by trypsin. Peptides were measured on an Orbitrap Astral mass spectrometer. Raw data files were processed by MaxQuant software package (version 2.6.2.0) using its integrated Andromeda search engine. Spectral data were searched against a target-decoy database consisting of the forward and reverse sequences of the WormPep release WS292 (28,587 entries), the UniProt E. coli K-12 reference proteome (release 2022_03; 4,460 entries), a list of N-terminally truncated WAGO-1/WAGO-3 proteins, WAGO-3 (P2G, A3E) mutant and a default list of common contaminants. False discovery rate (FDR) was set to 1% at both peptide and protein levels. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-09-08 |
| AnnouncementXML | Submission_2026-09-08_02:50:58.835.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | J Chen |
| SpeciesList | scientific name: Caenorhabditis elegans; common name: roundworm; NCBI TaxID: 6239; |
| ModificationList | iodoacetamide - site C |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-14 06:02:31 | ID requested | |
| ⏵ 1 | 2026-09-08 02:50:59 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: C. elegans, argonaute, N-terminus, WAGO-1, WAGO-3, DatasetType:Proteomics |
Contact List
| Rene Ketting | |
|---|---|
| contact affiliation | Institute of Molecular Biology, Mainz |
| contact email | R.Ketting@imb-mainz.de |
| lab head | |
| J Chen | |
| contact affiliation | Institute of Molecular Biology |
| contact email | j.chen@imb-mainz.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099904/ |
