⮝ Full datasets listing
PXD070705
PXD070705 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of human coronavirus 229E infected immortalized human lung fibroblasts (MRC5) at 8, 16, and 24 hours post infection (whole cell lysate) |
| Description | PISA assays performed on mock-infected and human coronavirus 229E-infected immortalized human lung fibroblasts (MRC5) cells (BTOL0001590). Samples were harvested at 8, 16, and 24 hours post infection (HCoV-299E). Samples are derived from human whole cell lysates (BTO:0004304). Mock-infected and human coronavirus 229E-infected immortalized human lung fibroblasts (MRC5) cells were collected at 8, 16, and 24 hours post infection (HCoV-299E). Cells were lysed by freeze thaw cycles and each replicate was split in half and treated for 1 minute with proteinase K and then trypsin and the other half just treated with trypsin prior to processing for proteomic analysis. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-19 |
| AnnouncementXML | Submission_2025-11-19_06:55:57.887.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Matthew Monroe |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Human coronavirus 229E; NCBI TaxID: 11137; |
| ModificationList | Oxidation; Carbamidomethyl; TMTpro |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-13 17:51:00 | ID requested | |
| ⏵ 1 | 2025-11-19 06:55:58 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: MRC5, PISA, DatasetType:Proteomics |
Contact List
| John T. Melchior | |
|---|---|
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | john.melchior@pnnl.gov |
| lab head | |
| Amy Sims | |
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | amy.sims@pnnl.gov |
| lab head | |
| Matthew Monroe | |
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | matthew.monroe@pnnl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099899/ |
