PXD070694

PXD070694 is an original dataset announced via ProteomeXchange.

Title	Kidney matrix dynamics across the life course
Description	Chronic kidney disease (CKD) represents a major public health concern and accounts for massive morbidity, mortality, and financial resource. Advanced age is a major risk factor for progressive CKD and is associated with kidney fibrosis. Dysregulated protein turnover is a hallmark of ageing and leads to matrix accumulation and abnormal matrix remodelling. As there are no current effective therapies to prevent or reverse kidney fibrosis, there is an urgent need to understand mechanisms that drive aberrant kidney matrix remodelling that occurs during ageing. Using our established experimental workflow for global quantification of protein half-lives in the kidney, we pulse-labelled mice with 13C-Lysine and performed deep proteomic profiling across four time-points which spanned the physiological life course of mice. This approach enabled us to define the natural dynamics of protein turnover in the kidney and specifically examine the influence of development and ageing on kidney matrix dynamics. This study provides novel insights into the mechanisms of matrix deposition during kidney maturation and begins to unravel the influence of physiological ageing on abnormal kidney matrix remodelling and accumulation. We identify several age-related proteolytic cascades which become dysregulated with age. Targeting early fibrotic remodelling of kidney matrix could help ameliorate inflammation and serve as a powerful tool in combating subsequent progression of age-related CKD.
HostingRepository	PRIDE
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_04:16:47.620.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Hoyle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-13 12:27:02	ID requested
⏵ 1	2026-05-21 04:16:48	announced

10.1016/J.MBPLUS.2026.100197;

submitter keyword: ECM, ageing, turnover,Kidney

Rachel Lennon
contact affiliation	Manchester Cell-Matrix Centre, Division of Cell-Matrix Biology and Regenerative Medicine, The University of Manchester, UK
contact email	Rachel.Lennon@manchester.ac.uk
lab head
Anna Hoyle
contact affiliation	University of Oxford
contact email	anna.hoyle@kennedy.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD070694

PRIDE project URI

• PRIDE
  1. PXD070694
     1. Label: PRIDE project
     2. Name: Kidney matrix dynamics across the life course