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PXD070689
PXD070689 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Data for Transcriptomic and Proteomic Signatures Following AS03-adjuvanted Influenza A/H7N9 Vaccine |
| Description | Vaccines targeting avian influenza virus A/H7N9 are poorly immunogenic. While the immune responses can be improved with oil-in-water emulsion adjuvants such as Adjuvant System 03 (AS03), the cellular mechanisms underpinning the adjuvant effect are incompletely characterized and poorly understood. In this study, we used RNA-sequencing and quantitative proteomics to elucidate the response to two doses of influenza A/H7N9 vaccine, with and without AS03 in six immune cell types. These responses were also compared to those seen after administration of an unadjuvanted seasonal influenza A/H3N2 variant vaccine to identify signatures unique to adjuvanted influenza vaccine and correlated with later antibody responses. Transcriptomic and proteomic analyses revealed that AS03-adjuvanted vaccine uniquely led to upregulation of immune pathways in innate immune cells within 24 hours following vaccination for phagocytosis, antigen presentation and processing, inflammasome activation, NK-cell mediated cytotoxicity, IgA production, and interferon-response pathways. Moreover, while major histocompatibility complex (MHC I and II) upregulation was observed across multiple immune cell types, the neutrophils showed a distinct activation of MHC II, generating the hypothesis that neutrophils may play a more important role in antigen presentation than previously understood. Taken together, these data provide a more complete mechanistic understanding of oil-in-water adjuvants and their role in enhancing the immune response for pandemic influenza preparedness. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-07-29 |
| AnnouncementXML | Submission_2026-07-29_10:42:39.601.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Brandon Hoenig |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; TMT; Oxidation |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-13 08:46:10 | ID requested | |
| ⏵ 1 | 2026-07-29 10:42:40 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Influenza, Influenza vaccine, H7N9, avian influenza, adjuvants, AS03, transcriptomics, proteomics, systems biology, DatasetType:Proteomics |
Contact List
| Clarence Creech, MD, MPH | |
|---|---|
| contact affiliation | Vanderbilt University Medical Center |
| contact email | buddy.creech@vumc.org |
| lab head | |
| Brandon Hoenig | |
| contact affiliation | The Emmes Company |
| contact email | bhoenig@emmes.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099890/ |
