PXD070664

PXD070664 is an original dataset announced via ProteomeXchange.

Title	Proteomics of FACS-sorted T cells from EMT-6 tumors
Description	This dataset contains data-independent acquisition (DIA) proteomic profiles of CXCR6+ and CXCR6− CD8+ T cells sorted from EMT-6 tumors by fluorescence-activated cell sorting (BD FACSAria III). Approximately 5,000 viable cells are collected per sample and cryopreserved prior to processing using a single-pot, miniaturized in-solution digestion workflow optimized for low-input samples. Peptides are analyzed by nanoLC coupled to a timsTOF Pro mass spectrometer (Bruker) with ion mobility separation. DIA data are processed in Spectronaut with dynamic iRT, MS2 interference correction, and cross-run normalization; results are filtered at a Q-value threshold of 0.01 (FDR < 1%). Downstream analyses, including differential protein abundance and functional enrichment, are performed in R (v4.3.1), and protein domain annotations are supported by InterProScan (Pfam).
HostingRepository	iProX
AnnounceDate	2025-11-13
AnnouncementXML	Submission_2026-01-11_23:01:06.170.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ming Yi
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-12 18:41:53	ID requested
⏵ 1	2026-01-11 23:01:06	announced

Dataset with its publication pending

submitter keyword: Cancer immunotherapy

Ming Yi
contact affiliation	Zhejiang University
contact email	mingyi_2022@zju.edu.cn
lab head
Ming Yi
contact affiliation	Zhejiang University
contact email	1978135000@qq.com
dataset submitter

iProX dataset URI