PXD070662

PXD070662 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of six AGS cell samples with low WDHD1 expression
Description	Tryptophan–aspartate repeat-containing and high mobility group box DNA-binding protein 1 (WDHD1), a key component of the replisome complex, functions as a trimeric regulator that orchestrates multiple steps of DNA replication. As an oncogenic factor, WDHD1 is aberrantly overexpressed in various types of cancers; however, its role in cancer biology, particularly in gastric cancer, remains largely unexplored. In this study, we comprehensively analyzed multi-omics datasets integrating TCGA, GTEx, TIMER2, GDSC, CTRP, and TISCH databases to elucidate the correlations between WDHD1 expression and clinical stage, diagnostic performance, and prognosis across different cancer types. We further investigated the relationships between WDHD1 expression, genetic alterations, methylation status, tumor stemness, and tumor immunity, and validated its prognostic value using the GEO database. Upstream transcription factors of WDHD1 were predicted using seven databases, and its potential mechanisms were explored by GO and KEGG enrichment analyses. Single-cell RNA-seq datasets were used to assess immune infiltration and cellular localization of WDHD1. Functional experiments including qRT-PCR, Western blotting, CCK-8, colony formation, and EdU assays were performed to determine its biological roles in gastric cancer cells. Pan-cancer analysis revealed that WDHD1 is mainly localized in the nucleus and significantly upregulated across multiple tumor types. Its expression was positively associated with higher tumor grade and stage in several cancers, showing moderate to high diagnostic accuracy. GO and KEGG analyses indicated that WDHD1 is strongly associated with cell proliferation and replication stress–related pathways. WDHD1 expression was negatively correlated with the majority of immune-related genes, suggesting that it acts as an immune-suppressive biomarker. Further analysis in gastric cancer identified GATA1 as a potential upstream transcription factor regulating WDHD1, which may promote tumor progression by driving cell-cycle progression and DNA replication. Single-cell expression profiling confirmed that WDHD1 is highly expressed in malignant epithelial cells, implying that its primary role lies in tumor cell proliferation and genome replication rather than immune modulation. In vitro experiments further demonstrated that WDHD1 facilitates gastric cancer cell proliferation. Collectively, our study identifies WDHD1 as a promising biomarker for cancer diagnosis, prognosis, and therapeutic targeting, highlighting its potential as a novel molecular driver of gastric cancer progression.
HostingRepository	iProX
AnnounceDate	2025-11-13
AnnouncementXML	Submission_2025-11-12_18:37:21.757.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xunkang Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-12 18:36:59	ID requested
⏵ 1	2025-11-12 18:37:22	announced

Dataset with its publication pending

submitter keyword: WD repeat and HMG-box DNA binding protein 1, Gastric cancer

Xiaoqiang Yue
contact affiliation	Department of Traditional Chinese Medicine, Changzheng Hospital, Naval Medical University
contact email	yuexiaoqiang@163.com
lab head
Xunkang Wang
contact affiliation	Department of Traditional Chinese Medicine, Changzheng Hospital, Naval Medical University, Shanghai, China
contact email	19921262115@163.com
dataset submitter

iProX dataset URI