PXD070661

PXD070661 is an original dataset announced via ProteomeXchange.

Title	Integrated Proteomic and Metabolomic Profiling Reveals Potential Virulence Factors and Secondary Metabolites in the Secretome of Fusarium verticillioides
Description	Fusarium verticillioides (F. verticillioides) is an important fungal pathogen known to infect a variety of economically critical crops, especially maize, causing substantial yield reductions and economic losses worldwide. In addition to its direct damage to agricultural productivity, F. verticillioides threatens public health through producing/secreting potent compounds, including the well-known fumonisins (FUMs), which pose significant health threats to both livestock and humans due to their toxicity and carcinogenicity. However, current knowledge about the materials secreted/produced by F. verticillioides, such as secreted proteins and additional secondary metabolites remains limited. In the present study, we conducted an integrated secretome analysis of F. verticillioides at exponential growth stage by using proteomic and metabolomic technologies. The results of the present study showed that proteomic analysis identified 185 proteins, including 138 fungus-specific proteins, GO enrichment of these 138 fungus-specific proteins yielded 24 significant terms spanning carbohydrate/polysaccharide and aminoglycan metabolic/catabolic processes, extracellular and membrane-anchored components, and hydrolase/peptidase activities. Meanwhile, KEGG analysis identified starch and sucrose metabolism as the sole significantly enriched pathway. Metabolomic analysis of medium supernatant showed that a total of 2,352 metabolites were identified, with 110 unique to the medium supernatant of fungal group, including fumonisins (A1, B2, B3, B4), fatty acids, and other bioactive compounds. KEGG pathway enrichment highlighted key metabolic pathways such as TCA cycle, unsaturated fatty acid biosynthesis, and arachidonic acid metabolism. These findings provide new insights into the pathogenic mechanisms of F. verticillioides, suggesting potential virulence factors and metabolic adaptations that contribute to its pathogenicity.
HostingRepository	iProX
AnnounceDate	2025-11-12
AnnouncementXML	Submission_2025-11-12_18:34:55.579.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Minmin Sui
SpeciesList	scientific name: Fusarium verticillioides; NCBI TaxID: 117187;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-12 18:34:35	ID requested
⏵ 1	2025-11-12 18:34:56	announced

Dataset with its publication pending

submitter keyword: Fusarium verticillioides, fumonisins, secretions, proteomic, metabolomic

Junjun He
contact affiliation	Faculty of Animal Science and Technology, Yunnan Agricultural University
contact email	hejunjun617@163.com
lab head
Minmin Sui
contact affiliation	Faculty of Animal Science and Technology, Yunnan Agricultural University
contact email	1454892881@qq.com
dataset submitter

iProX dataset URI