PXD070644

PXD070644 is an original dataset announced via ProteomeXchange.

Title	Structure of NO16, a marine non-tailed vibriophage with an unusual symmetry-mismatched vertex arrangement
Description	Non-tailed phages remain underexplored in marine environments, as tailed phages have long dominated sequence and culture collections. Yet recent surveys suggest that non-tailed phages may be more abundant and have distinct impacts on microbial mortality and gene transfer. Here, we solve the structure of Vibrio anguillarum bacteriophage NO16, one of the simplest members of the Varidnaviria realm. Mass spectrometry detected at least nine different proteins in the virion, which has a pseudoT=21 capsid similar to that of related double jelly roll (DJR) phages, but differs in the organization of minor capsid proteins, particularly those mediating membrane–capsid contacts. The DJR major capsid protein GP19 is stabilized by strong electrostatic interactions between monomers, and possibly by a cation at its base, as seen in corticovirus PM2. Localized reconstruction revealed a symmetry mismatch at the vertex, where two trimeric GP13 spikes attach to the pentameric GP14 penton base. GP13 carbohydrate-binding sites and predicted glycosylase activity point to a role in host entry. Using structural and functional predictions for its entire proteome, we propose a complete atlas of the NO16 infectious cycle.
HostingRepository	PRIDE
AnnounceDate	2026-08-07
AnnouncementXML	Submission_2026-08-07_02:07:23.147.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Otaegi Ugartemendia
SpeciesList	scientific name: Elsinorevirus NO16; NCBI TaxID: NEWT:3428464; scientific name: Vibrio anguillarum; NCBI TaxID: NEWT:55601;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-12 11:54:27	ID requested
⏵ 1	2026-08-07 02:07:23	announced

10.1371/JOURNAL.PPAT.1014525;

submitter keyword: vibriophage, marine virus

Carmen San Martin
contact affiliation	Centro Nacional de Biotecnología (CNB-CSIC)
contact email	carmen@cnb.csic.es
lab head
Sara Otaegi Ugartemendia
contact affiliation	National Centre for Biotechnology (CNB-CSIC)
contact email	sotaegi@cnb.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD070644

PRIDE project URI

• PRIDE
  1. PXD070644
     1. Label: PRIDE project
     2. Name: Structure of NO16, a marine non-tailed vibriophage with an unusual symmetry-mismatched vertex arrangement