PXD070632

PXD070632 is an original dataset announced via ProteomeXchange.

Title	Unraveling the matrix: Proteomic profiling reveals stromal ECM dysregulation in severe early-onset fetal growth restriction
Description	Severe, early-onset fetal growth restriction with absent or reversed end-diastolic umbilical artery velocities (FGRa/r) is marked by profound placental vascular insufficiency, yet the role of the villous stromal extracellular matrix (ECM) in this pathology remains poorly defined. Here, we applied an ECM-optimized proteomic workflow to villous tissue and fibroblast-derived cell matrices (CDMs) from FGRa/r, gestational age–matched preterm controls, and uncomplicated term placentas. While villous tissue exhibited only subtle trends toward increased type I collagen (COL1A1/2) and fibronectin (FN1), CDMs revealed a distinct FGRa/r signature characterized by elevated total matrisome abundance, greater insolubility of matrisome-associated proteins, and 44 differentially expressed insoluble ECM proteins. Fibronectin emerged as a central network hub, interacting with thrombospondin-1 (THBS1), vitronectin (VTN), and transglutaminase-2 (TGM2), all of which were enriched in FGRa/r CDM, suggesting excessive deposition and crosslinking. In contrast, regulators of ECM remodeling and TGFβ activity, including fibrillin-1 (FBN1), decorin (DCN), and syndecan-4 (SDC4), were depleted. These features define a pro-fibrotic, dysregulated stromal microenvironment with diminished remodeling capacity and altered basement membrane composition. Together, our findings establish the first comprehensive proteomic map of the human placental stromal matrisome in FGRa/r, highlight the ECM as a critical regulator of angiogenic competence, and provide a molecular framework for understanding how aberrant ECM organization contributes to placental dysfunction.
HostingRepository	PRIDE
AnnounceDate	2025-12-08
AnnouncementXML	Submission_2025-12-08_04:58:50.960.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kirk Hansen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-12 03:01:53	ID requested
⏵ 1	2025-12-08 04:58:52	announced

10.3390/ijms262211179;

Ginocchio S, McCabe MC, Flockton AR, Gumina DL, Hansen KC, Ji S, Su EJ, Unraveling the Matrix: Proteomic Profiling Reveals Stromal ECM Dysregulation in Severe Early-Onset Fetal Growth Restriction. Int J Mol Sci, 26(22):(2025) [pubmed]

submitter keyword: proteomics, fetal growth restriction, pregnancy, fibrosis, collagen,extracellular matrix, placenta

Emily Su
contact affiliation	University of Colorado Anschutz Medical Campus Obstetrics and Gynecology
contact email	emily.su@cuanschutz.edu
lab head
Kirk Hansen
contact affiliation	Univeristy of Colorado
contact email	kirk.hansen@cuanschutz.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070632

PRIDE project URI

• PRIDE
  1. PXD070632
     1. Label: PRIDE project
     2. Name: Unraveling the matrix: Proteomic profiling reveals stromal ECM dysregulation in severe early-onset fetal growth restriction