⮝ Full datasets listing
PXD070618
PXD070618 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PCK2-mediated PQBP1 lactylation promotes asthmatic inflammation through PRMT5 inhibition |
| Description | Background: Asthma involves chronic inflammation linked to metabolic reprogramming, but how metabolites reshape epigenetics through post-translational modifications remains unclear. Methods: We used HDM-induced asthmatic mice with multi-omics analyses (metabolomics, PTM-proteomics, ChIP-seq) and validated findings through gene editing and AAV interventions. Results: Asthmatic airways showed lactate-driven glutaminolysis, causing lactate/succinate accumulation. PCK2 succinylation at K100 enhanced stability by antagonizing ubiquitination, creating a lactate-generating feedback loop. Accumulated lactate triggered PQBP1 lactylation at K223, enabling PRMT5/WDR77 complex inhibition. This erased H4R3me2s repressive marks from pro-inflammatory gene promoters, particularly MAPK pathway genes, causing transcriptional de-repression. Airway epithelium-specific PQBP1 knockout reduced inflammation, goblet cell hyperplasia, and Th2 responses. PCK2-shRNA or oxamate treatment ameliorated asthmatic pathology. Conclusion: We identified a PCK2-lactate-PQBP1-PRMT5 axis linking metabolic reprogramming to epigenetic dysregulation in asthma. PCK2-K100 succinylation drives lactate accumulation, inducing PQBP1-K223 lactylation that inhibits PRMT5 and activates inflammatory genes, representing a therapeutic target for asthma. |
| HostingRepository | iProX |
| AnnounceDate | 2025-11-12 |
| AnnouncementXML | Submission_2025-11-11_19:05:35.975.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Guanghai Yan |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-11 19:05:10 | ID requested | |
| ⏵ 1 | 2025-11-11 19:05:36 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Asthma, Lactylation, Succinylation |
Contact List
| Guanghai Yan | |
|---|---|
| contact affiliation | Department of Anatomy, Histology and Embryology, Yanbian University Medical College |
| contact email | ghyan@ybu.edu.cn |
| lab head | |
| Guanghai Yan | |
| contact affiliation | Department of Anatomy, Histology and Embryology, Yanbian University Medical College |
| contact email | ghyan2015@sina.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
