PXD070596

PXD070596 is an original dataset announced via ProteomeXchange.

Title	Unravelling the molecular responses of the yeast Schwanniomyces etchellsii to hyperosmotic stress in seawater medium using omic approaches. Experiments 2 and 4
Description	Schwanniomyces etchellsii is an unconventional, halotolerant microorganism. Like some other yeasts, it can efficiently perform various biocatalytic transformations of organic compounds in seawater more effectively than in freshwater. In seawater, conversion rates are higher, by-product production is minimized, greater substrate loading is possible, and cells can be recycled for further use. To identify the molecular features that explain this behavior, comparative proteomic and lipidomic studies were conducted on cells grown in seawater and freshwater at various growth stages. The results showed higher expression of proteins involved in the stress response, such as glycerol-3-phosphate dehydrogenase, the glycerol transporter Stl1 and the P-type ATPase sodium pump Ena1, and several phospholipid biosynthesis proteins, including inositol-3-phosphate synthase and phosphatidate cytidylyltransferase, in seawater. Changes in metabolic enzymes and other proteins involved in responding to stimuli were also observed between the two conditions. Overall, cells grown in a freshwater medium exhibited higher levels of enzymes involved in biosynthetic processes. Differences in lipid profiles were also observed between cells grown in the two media. Higher levels of monoacyl and diacylglycerols were found in seawater, while higher levels of phospholipids containing serine and ethanolamine were found in freshwater. Consistent with more permeable membranes, cells grown in seawater exhibited lower levels of ergosterol.
HostingRepository	PRIDE
AnnounceDate	2026-01-19
AnnouncementXML	Submission_2026-01-18_16:26:02.690.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Schwanniomyces etchellsii; NCBI TaxID: NEWT:28550;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600+

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-11 10:39:05	ID requested
⏵ 1	2026-01-18 16:26:03	announced

10.3390/ijms27010183;

Andreu C, Obis È, Del Olmo ML, to Hyperosmotic Stress in Seawater Medium Using Omic Approaches. Int J Mol Sci, 27(1):(2025) [pubmed]

submitter keyword: Schwanniomyces etchellsii, comparative proteomics, hyperosmotic stress

Marcel·lí del Olmo
contact affiliation	Departament de Bioquímica i Biologia Molecular Universitat de València
contact email	M.Del.Olmo@uv.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD070596

PRIDE project URI

• PRIDE
  1. PXD070596
     1. Label: PRIDE project
     2. Name: Unravelling the molecular responses of the yeast Schwanniomyces etchellsii to hyperosmotic stress in seawater medium using omic approaches. Experiments 2 and 4