PXD070575

PXD070575 is an original dataset announced via ProteomeXchange.

Title	Proteomics of A549 and A549 cells transducted with ORF7a SARS-CoV-2 accessory protein
Description	SARS-CoV-2 reprograms host metabolism to facilitate immune evasion and viral replication. While infection is known to impair mitochondrial function and enhance glycolysis, the role of viral accessory proteins in these alterations remains unclear. Here, we investigate the metabolic impact of the accessory protein ORF7a. Using lentiviral transduction, we expressed ORF7a in human lung epithelial (A549) and monocytic (THP1) cells. Integrated transcriptomic, proteomic, and metabolomic analyses in A549 cells revealed profound dysregulation of glucose and lipid metabolism. ORF7a impaired oxidative phosphorylation, as shown by reduced basal and maximal respiration, mitochondrial depolarization, and elevated reactive oxygen species. ORF7a induced upregulation of pyruvate dehydrogenase kinase 4 (PDK4) and increased phosphorylation of the pyruvate dehydrogenase complex (PDHC), leading to impaired pyruvate oxidation. However, pharmacological inhibition of PDK4 with dichloroacetate failed to rescue mitochondrial respiration, suggesting additional dysfunction. Enzymatic assays confirmed reduced complex I activity, pointing to direct impairment of the electron transport chain. Our findings demonstrate that ORF7a orchestrates a multifaceted metabolic reprogramming involving PDK4-dependent PDHC inhibition and complex I dysfunction. These results highlight ORF7a as a key modulator of host metabolism and underscore mitochondrial pathways as potential therapeutic targets in COVID-19.
HostingRepository	PRIDE
AnnounceDate	2026-07-08
AnnouncementXML	Submission_2026-07-08_06:31:56.010.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD070575
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Raúl Fernández Rodríguez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-10 23:41:07	ID requested
⏵ 1	2026-07-08 06:31:56	announced

10.6019/PXD070575;

submitter keyword: SARS-CoV-2

accessory proteins

ORF7a

COVID-19

Tránsito García García
contact affiliation	Department of Genetics, Immunogenomics and Molecular Pathogenesis Group, UIC Zoonoses and Emergent Diseases ENZOEM, University of Córdoba, Córdoba, Spain
contact email	b52gagat@uco.es
lab head
Raúl Fernández Rodríguez
contact affiliation	Department of Genetics, Immunogenomics and Molecular Pathogenesis Group, UIC Zoonoses and Emergent Diseases ENZOEM, University of Córdoba, Córdoba, Spain.
contact email	b42feror@uco.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070575
     1. Label: PRIDE project
     2. Name: Proteomics of A549 and A549 cells transducted with ORF7a SARS-CoV-2 accessory protein