PXD070546

PXD070546 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic characterization of human iPSC-derived cardiomyocytes
Description	Cardiomyocytes differentiated in vitro from human induced pluripotent stem cells (iPSC-CMs) are increasingly used in studies of disease mechanisms, drug development, toxicity testing, and regenerative medicine. However, the extent to which iPSC-CMs recapitulate native cardiac gene expression programs, at both the splicing and protein levels, remains poorly understood. We investigated the alternative splicing profiles of in vitro differentiated iPSC-CMs in comparison to fetal and adult heart samples. To examine whether splicing isoforms identified in RNA-seq datasets give rise to proteins, we generated a comprehensive proteomic dataset of iPSC-CMs. Integration of RNA and protein datasets enabled the detection of isoform-specific peptides, demonstrating translation of selected alternatively spliced transcripts.
HostingRepository	PRIDE
AnnounceDate	2025-12-30
AnnouncementXML	Submission_2025-12-30_03:26:12.308.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD070546
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	André Ventura-Gomes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-10 08:03:56	ID requested
⏵ 1	2025-12-30 03:26:12	announced

Publication List

10.1016/J.STEMCR.2025.102757;
10.6019/PXD070546;

10.1016/J.STEMCR.2025.102757;

10.6019/PXD070546;

Keyword List

submitter keyword: None

submitter keyword: None

Contact List

Carmo Fonseca
contact affiliation	GIMM – Gulbenkian Institute for Molecular Medicine
contact email	carmo.fonseca@gimm.pt
lab head
André Ventura-Gomes
contact affiliation	GIMM – Gulbenkian Institute for Molecular Medicine
contact email	andre.v.gomes@gimm.pt
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070546
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070546

PRIDE project URI

Repository Record List

[ + ]