PXD070488

PXD070488 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comprehensive N-glycoproteomic profiling of humanized antibody expressed in transgenic mouse serum
Description	This dataset reports a comprehensive N-glycoproteomic profiling of serum-derived humanized antibodies expressed in transgenic mice using an intact glycopeptide (IGP)–based LC–MS/MS workflow. The study aimed to identify and characterize site-specific N-glycosylation modifications of mouse serum proteins, with emphasis on antibody-related glycoforms. Following sample digestion and HILIC enrichment, glycopeptides were analyzed by high-resolution Orbitrap Astral mass spectrometry in data-dependent acquisition (DDA) mode. The identified N-linked glycopeptides were annotated for glycan composition, glycosylation heterogeneity, and functional classification through GO, KEGG, Reactome, and STRING analyses. The dataset provides an extensive resource for understanding host-dependent glycosylation patterns and their potential impact on antibody functionality.
HostingRepository	PRIDE
AnnounceDate	2025-12-08
AnnouncementXML	Submission_2025-12-07_16:30:34.306.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiyang Jiang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-08 07:00:51	ID requested
⏵ 1	2025-12-07 16:30:35	announced

Publication List

Jiang Z, Jia B, Hu N, Zhang M, Xiao H, Chen G, Yu J, Li X, Shen B, Feng J, Wang J, engineering of transgenic mice for systemic human neutralizing antibody production against staphylococcal enterotoxin B. Front Immunol, 16():1679421(2025) [pubmed]
10.3389/fimmu.2025.1679421;

Jiang Z, Jia B, Hu N, Zhang M, Xiao H, Chen G, Yu J, Li X, Shen B, Feng J, Wang J, engineering of transgenic mice for systemic human neutralizing antibody production against staphylococcal enterotoxin B. Front Immunol, 16():1679421(2025) [pubmed]

10.3389/fimmu.2025.1679421;

Keyword List

submitter keyword: antibody, transgenic mouse, post-translational modification, glycoproteomics, Orbitrap Astral,N-glycosylation, intact glycopeptides, DDA, LC–MS/MS

submitter keyword: antibody, transgenic mouse, post-translational modification, glycoproteomics, Orbitrap Astral,N-glycosylation, intact glycopeptides, DDA, LC–MS/MS

Contact List

Jing Wang
contact affiliation	State Key Laboratory of National Security Specially Needed Medicines, Beijing 100039, China.
contact email	Jingw_biomed@163.com
lab head
Zhiyang Jiang
contact affiliation	Academy of Military Medical Sciences
contact email	2292737488@qq.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070488
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070488

PRIDE project URI

Repository Record List

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