PXD070479

PXD070479 is an original dataset announced via ProteomeXchange.

Title	Exoproteome remodeling by the BraI/R Quorum Sensing circuit and interspecies competition in Paraburkholderia
Description	Quorum sensing (QS) is a cell-to-cell communication mechanism that enables bacteria to coordinate collective behaviors in response to population density. The BraI/R QS system is conserved among Burkholderia and Paraburkholderia species, and its regulatory role is still unknown. In this study, the BraI/R QS of P. kururiensis was investigated for biological control of another bacterial species. The results demonstrate that the functional BraI/R system influences interbacterial competition, as braI and braR mutant strains failed to inhibit the growth of Escherichia coli, in contrast to the wild-type strain. These findings suggest that the BraI/R QS system plays a regulatory role in modulating interspecies antagonism. Comparative exoproteome analyses were performed for the wild-type and the BraI/R QS-mutant P. kururiensis strains, revealing distinct protein populations, including structural and effector proteins of the Type VI secretion system (T6SS). Genomic mapping revealed that two of those T6SS-proteins were encoded within a specific T6SS gene cluster (T6SS-3), while others originate outside any major T6SS gene cluster. The identification of exoproteome targets regulated by the BraI/R QS system represents a significant advance toward the understanding of the molecular mechanisms underpinning P. kururiensis interactions with competing bacteria, further supporting its potential as a biological control agent against phytopathogenic and/or free-living species.
HostingRepository	PRIDE
AnnounceDate	2026-07-29
AnnouncementXML	Submission_2026-07-29_11:26:19.918.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Unit
SpeciesList	scientific name: Paraburkholderia kururiensis M130; NCBI TaxID: NEWT:1268285;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-07 15:23:51	ID requested
⏵ 1	2026-07-29 11:26:20	announced

10.1002/PMIC.70163;

submitter keyword: Paraburkholderia, Type VI Secretion System, Quorum Sensing, Proteomics, Biological Control.

Fábio C. S. Nogueira
contact affiliation	Institute of Chemistry, Federal University of Rio de Janeiro, Brazil.
contact email	fabiocsn@iq.ufrj.br
lab head
Proteomics Unit
contact affiliation	UFRJ
contact email	unidadeproteomica@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070479
     1. Label: PRIDE project
     2. Name: Exoproteome remodeling by the BraI/R Quorum Sensing circuit and interspecies competition in Paraburkholderia