PXD070428

PXD070428 is an original dataset announced via ProteomeXchange.

Title	Proteomic profile of (Laser-dissected) Motoneurons, Ependymal Cells and Dorsal Root Ganglia Cells, and after Spinal Cord Injury in Rat
Description	Spinal cord injury triggers extensive molecular remodeling in neural and glial compartments. To resolve these processes at cell-type-specific resolution, we performed quantitative proteomic profiling of laser-microdissected motor neurons (MNs), dorsal root ganglia (DRG), and ependymal cells (EC) from control Wistar rats and 2 or 6 weeks after complete spinal cord transection at Th10. Retrogradely traced MNs innervating the soleus (SOL; 172 ± 39 cells) and tibialis anterior (TA; 262 ± 74 cells) muscles, ECs from the central canal (L3–L6) were microdissected from spinal cord slices with Leica LMD7000 Laser Microdissection System. Bilateral DRG (L3–L5) were also isolated. Samples from five animals per group were analyzed using TMT-based LC–MS/MS, yielding 1 221 proteins in SOL MNs, 1 186 in TA MNs, 1,520 in ECs, and 5 087, 3 740, and 3 086 in DRG L3, L4, and L5, respectively. The dataset supports cell-type-specific characterization of molecular responses after spinal injury.
HostingRepository	PRIDE
AnnounceDate	2026-03-13
AnnouncementXML	Submission_2026-03-13_04:26:07.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-06 17:43:03	ID requested
⏵ 1	2026-03-13 04:26:07	announced

Dataset with its publication pending

submitter keyword: Motorneurons, Dorsal root ganglia,Spinal cord injury, Laser microdissection, TMT-based proteomics

Olga Gajewska-Woźniak
contact affiliation	Nencki Institute of Experimental Biology, Warsaw, Poland
contact email	o.gajewska@nencki.edu.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD070428

PRIDE project URI

• PRIDE
  1. PXD070428
     1. Label: PRIDE project
     2. Name: Proteomic profile of (Laser-dissected) Motoneurons, Ependymal Cells and Dorsal Root Ganglia Cells, and after Spinal Cord Injury in Rat