PXD070419

PXD070419 is an original dataset announced via ProteomeXchange.

Title	T-cell derived exosomes released from Multi-Core Granules exhibit potent caspase 3 mediated tumor suppressive activity ( Part II)
Description	Small extracellular vesicles (sEV) derived from cytotoxic T lymphocytes (CTLs) are emerging as potential mediators of antitumor immunity, yet their subcellular origins and functional properties remain incompletely defined. In this study, we investigated the intracellular routes and cytotoxic potential of CTL-derived sEVs. Using correlative light and electron microscopy, we discovered that CTL-derived sEVs are exosomes, as they originate from both the classical multivesicular bodies (MVBs) and the recently identified multicore granules (MCGs). Applying total internal reflection fluorescence microscopy, we revealed that, in contrast to MVB-derived exosomes, MCG-derived exosomes are released at the immunological synapse in a stimulus-dependent manner. To enable functional characterization, we developed a scalable primary cell culture method for the isolation of high-purity exosomes. Super-resolution microscopy demonstrated significant heterogeneity in exosome size and tetraspanin composition. Notably, MCG-derived exosomes demonstrated five times higher cytotoxic activity than MVB-derived exosomes, inducing apoptosis in tumor cells via a caspase-3-dependent mechanism. These findings reveal that CTLs exploit distinct secretory pathways to release heterogeneous exosome populations with differential cytotoxic capacities, offering new insights into CTL-mediated immune responses and providing a basis for the development of novel exosome-based immunotherapies
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_17:33:54.765.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD070419
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Claudia Fecher-Trost
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-06 13:33:20	ID requested
⏵ 1	2026-02-22 17:33:55	announced

10.6019/PXD070419;

Alawar N, Schirra C, Rasuli R, Fecher-Trost C, Weins L, Meyer MR, Flockerzi V, Keller A, Dustin ML, Rettig J, Becherer U, T-Cell-Derived Exosomes From Multi Core Granules Exhibit Superior Caspase-3-Mediated Tumor-Suppressive Activity Compared to Those From Multivesicular Bodies. J Extracell Vesicles, 15(2):e70239(2026) [pubmed]

10.1002/jev2.70239;

submitter keyword: tetraspanins, 10x-expansion, Munc13-4, extracellular vesicles,primary cells, CD8+ T-cells, immunological synapse, multivesicular bodies

Ute Becherer
contact affiliation	Cellular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, 66421 Homburg, Germany
contact email	Ute.Becherer@uks.eu
lab head
Claudia Fecher-Trost
contact affiliation	PZMS
contact email	claudia.fecher-trost@uks.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD070419

PRIDE project URI

• PRIDE
  1. PXD070419
     1. Label: PRIDE project
     2. Name: T-cell derived exosomes released from Multi-Core Granules exhibit potent caspase 3 mediated tumor suppressive activity ( Part II)